SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network.
SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network.
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DOI：
10.1590/0074-02760200342
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发表时间：
2020
影响因子：
2.8
通讯作者：
Durigon EL
中科院分区：
文献类型：
--
作者：
Araujo DB;Machado RRG;Amgarten DE;Malta FM;de Araujo GG;Monteiro CO;Candido ED;Soares CP;de Menezes FG;Pires ACC;Santana RAF;Viana AO;Dorlass E;Thomazelli L;Ferreira LCS;Botosso VF;Carvalho CRG;Oliveira DBL;Pinho JRR;Durigon EL
巴西于2020年2月确诊了严重急性呼吸综合征冠状病毒2型（SARS-CoV-2），在出现第一例病例后，全国病例数不断增加，导致了一场重大的公共卫生危机，需要快速和协调的应对措施。本研究的目的是描述从巴西首例冠状病毒病2019 （COVID-19）确诊病例中分离出的SARS-CoV-2分离物的分离和传播特性。在2月底通过RT-PCR对从意大利返回<s:1>圣保罗市的患者进行诊断后，在细胞培养中获得了SARS-CoV-2分离株，并通过全基因组测序、电子显微镜和体外复制特性进行了表征。从鼻咽标本中回收病毒分离物，在Vero细胞（E6、CCL-81和hSLAM）中繁殖，具有明显的细胞病变作用，并通过全基因组测序、电子显微镜和体外复制特性进行了表征。病毒库-活病毒（滴度2.11 × 106 TCID50/mL，滴度1.5 × 106 pfu /mL），从SARS.CoV2/SP02.2020.HIAE分离株中灭活。已编制并提供给巴西和国外的公共卫生当局和科学界。我们认为，这里描述的病毒生长和研究规程以及分发倡议可能成为其他发展中国家的可行模式，不仅有助于迅速有效地应对大流行病，而且有助于促进和支持基础科学研究。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research.
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影响因子：
11.8
作者：
Kaye M
通讯作者：
Kaye M
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
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Ziebuhr, John
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Pevzner, Pavel A.
影响因子：
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作者：
Gillim-Ross, L;Taylor, J;Wentworth, DE
通讯作者：
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