Esterase from a cariogenic bacterium hydrolyzes dental resins.
Esterase from a cariogenic bacterium hydrolyzes dental resins.
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DOI：
10.1016/j.actbio.2018.02.020
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发表时间：
2018-04-15
影响因子：
9.7
通讯作者：
Finer Y
中科院分区：
文献类型：
--
作者：
Huang B;Siqueira WL;Cvitkovitch DG;Finer Y
鉴定和表征S.对基于甲基丙烯酸酯的树脂单体具有降解活性的变异蛋白。在几种推定的酯酶中，发现在开放阅读框中编码为SMU_118 c（国家生物技术信息中心，NCBI）的酯酶具有真正的水解酶活性。对SMU_118c进行克隆、表达、纯化，并进一步表征其在中性（7.0）或致龋性（5.5）pH下对含酯硝基苯底物和通用树脂单体双苯基甘氨酰二甲基丙烯酸酯（bisGMA）和三甘醇二甲基丙烯酸酯（TEGDMA）的水解活性。变异UA159.与S.在变形杆菌中，SMU_118c显示出对乙酸对硝基苯酯（pNPA）和丁酸对硝基苯酯（pNPB）相对于丁酸邻硝基苯酯（oNPB）和碘化丁酰硫代胆碱（BTC）的最高亲和力（p<0.05）。酯酶在21天后保留其活性的60%，并且在中性和致龋pH下以比TEGDMA更高的速率水解bisGMA（p<0.001），类似于主要的人唾液酯酶降解活性。MS分析证实SMU_118c为S.变形杆菌UA159，并在致病性（pH 5.5）生长条件下表达。活性曲线与整个S. SMU_118c在变形链球菌细胞中的稳定性、致龋pH下的稳定性、对bisGMA的水解偏好以及已证实的表达谱表明SMU_118c可能对变形链球菌的整个细菌降解活性有显著贡献。变形菌对树脂复合材料，粘合剂和牙齿界面的降解，潜在地加速修复体的失败。
To identify and characterize specific esterases from S. mutans with degradative activity toward methacrylate-based resin monomers. Out of several putative esterases, an esterase encoded in an Open Reading Frame as SMU_118c (The National Center for Biotechnology Information, NCBI), was found to have true hydrolase activities. SMU_118c was cloned, expressed, purified and further characterized for its respective hydrolytic activity towards ester-containing nitrophenyl substrates and the universal resin monomers bis-phenyl-glycidyl-dimethacrylate (bisGMA) and triethyleneglycol dimethacrylate (TEGDMA) at neutral (7.0) or cariogenic (5.5) pH. Mass spectrometry (MS) was used to verify the expression of SMU_118c protein in S. mutans UA159. Similar to the whole cell activity of S. mutans, SMU_118c showed the highest affinity toward p-nitrophenyl acetate (pNPA) and p-nitrophenyl butyrate (pNPB) vs. o-nitrophenyl butyrate (oNPB) and butyrylthiocholine iodide (BTC) (p<0.05). The esterase retained 60% of its activity after 21 days and hydrolyzed bisGMA at a higher rate than TEGDMA at both neutral and cariogenic pH (p<0.001), similarly to the predominant human salivary esterase degradative activity. MS confirmed that SMU_118c is an intracellular protein in S. mutans UA159 and expressed under pathogenic (pH 5.5) growth conditions. The similarity in the activity profile to the whole S. mutans bacterial cell, the stability over time at cariogenic pH, the preference to hydrolyze bisGMA and confirmed expression profile suggest that SMU_118c could be a significant contributor to the whole bacterial degradative activity of S. mutans toward the degradation of resin composites, adhesives and the restoration-tooth interface, potentially accelerating restoration’s failure.
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影响因子：
14
作者：
Lin, BA;Jaffer, F;Santerre, JP
通讯作者：
Santerre, JP
影响因子：
5.6
作者：
Bencharit, Sompop;Edwards, Carol C.;Redinbo, Matthew R.
通讯作者：
Redinbo, Matthew R.
影响因子：
5
作者：
Cai, Kuihua;Delaviz, Yasaman;Santerre, J. Paul
通讯作者：
Santerre, J. Paul
影响因子：
4.9
作者：
Finer, Y;Santerre, JP
通讯作者：
Santerre, JP
影响因子：
3.1
作者：
Matsui R;Cvitkovitch D
通讯作者：
Cvitkovitch D