Absolute Quantification of Nanoparticle Interactions with Individual Human B Cells by Single Cell Mass Spectrometry.
Absolute Quantification of Nanoparticle Interactions with Individual Human B Cells by Single Cell Mass Spectrometry.
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纳米粒子与单个人B细胞相互作用的单细胞质谱绝对定量。
DOI：
10.1021/acs.nanolett.2c01037
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发表时间：
2022-05-25
期刊：
影响因子：
10.8
通讯作者：
Wilhelm, Stefan
中科院分区：
文献类型：
--
作者：
Donahue, Nathan D.;Sheth, Vinit;Frickenstein, Alex N.;Holden, Alyssa;Kanapilly, Sandy;Stephan, Chady;Wilhelm, Stefan
我们报告使用基于四极杆的电感耦合等离子体质谱法（ICP-MS）的纳米粒子与单个人B细胞相互作用的绝对定量。该方法能够在单个纳米颗粒和单个细胞水平上定量纳米颗粒-细胞相互作用。我们证明了单独悬浮的B细胞的有效和准确的检测，并发现胶体稳定的带正电荷的纳米颗粒与单个B细胞的缔合比带中性电荷的纳米颗粒高约100倍。我们证实这些纳米颗粒被单个B细胞内化，并确定内化通过与内吞作用一致的能量依赖性途径发生。使用双分析物ICP-MS，我们确定> 80%的单个B细胞对纳米颗粒呈阳性。我们的研究展示了ICP-MS工作流程，用于对具有单细胞和单纳米颗粒分辨率的纳米颗粒-细胞相互作用进行绝对定量。这种独特的工作流程可以为控制细胞相互作用（包括免疫细胞-纳米颗粒相互作用）的各种纳米材料的合理设计提供信息。
We report on the absolute quantification of nanoparticle interactions with individual human B cells using quadrupole-based inductively coupled plasma mass spectrometry (ICP-MS). This method enables the quantification of nanoparticle-cell interactions at single nanoparticle and single cell levels. We demonstrated the efficient and accurate detection of individually suspended B cells and found a ~100-fold higher association of colloidally stable positively charged nanoparticles with single B cells than neutrally charged nanoparticles. We confirmed that these nanoparticles were internalized by individual B cells and determined that the internalization occurred via energy-dependent pathways consistent with endocytosis. Using dual analyte ICP-MS, we determined that >80% of single B cells were positive for nanoparticles. Our study demonstrated an ICP-MS workflow for the absolute quantification of nanoparticle-cell interactions with single cell and single nanoparticle resolution. This unique workflow could inform the rational design of various nanomaterials for controlling cellular interactions, including immune cell-nanoparticle interactions.
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