Mechanical control of neural plate folding by apical domain alteration.
Mechanical control of neural plate folding by apical domain alteration.
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DOI：
10.1038/s41467-023-43973-x
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发表时间：
2023-12-20
影响因子：
16.6
通讯作者：
Sokol, Sergei Y.
中科院分区：
文献类型：
--
作者：
Matsuda, Miho;Rozman, Jan;Ostvar, Sassan;Kasza, Karen E.;Sokol, Sergei Y.
脊椎动物神经管闭合与细胞形状和行为的复杂变化有关，然而，这些过程对组织折叠的相对贡献还不清楚。在非洲爪蟾神经管折叠的发病，我们观察到交替的顶部收缩和顶部扩大的细胞。这种顶端结构域的异质性伴随着偏向细胞取向沿着前后轴，特别是在神经板铰链，并需要平面细胞极性信号。顶点模型表明，分散的各向同性收缩细胞可以引起相邻细胞的伸长。同时，在外胚层中，细胞自主的顶端收缩伴随着相邻的扩张。因此，各向同性收缩细胞的子集可以启动神经板弯曲，而力产生细胞和响应细胞之间的“拔河”竞赛减少了其沿着体轴的收缩。这种机制是垂直于体轴的细胞连接的各向异性收缩的替代方案。我们建议，顶端域的变化反映了平面极性依赖的机械力在神经折叠过程中操作。理论和实验观察表明，神经板顶端结构域的异质性是收缩细胞和伸长细胞之间的拔河比赛。这种竞争可能反映了在组织弯曲期间操作的机械力。
Vertebrate neural tube closure is associated with complex changes in cell shape and behavior, however, the relative contribution of these processes to tissue folding is not well understood. At the onset of Xenopus neural tube folding, we observed alternation of apically constricted and apically expanded cells. This apical domain heterogeneity was accompanied by biased cell orientation along the anteroposterior axis, especially at neural plate hinges, and required planar cell polarity signaling. Vertex models suggested that dispersed isotropically constricting cells can cause the elongation of adjacent cells. Consistently, in ectoderm, cell-autonomous apical constriction was accompanied by neighbor expansion. Thus, a subset of isotropically constricting cells may initiate neural plate bending, whereas a ‘tug-of-war’ contest between the force-generating and responding cells reduces its shrinking along the body axis. This mechanism is an alternative to anisotropic shrinking of cell junctions that are perpendicular to the body axis. We propose that apical domain changes reflect planar polarity-dependent mechanical forces operating during neural folding. Theoretical and experimental observations argue that apical domain heterogeneity in the neural plate is a tug-of-war contest between constricted and elongated cells. This competition likely reflects mechanical forces operating during tissue bending.
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