Genomic and proteomic analyses of the terminally redundant genome of the Pseudomonas aeruginosa phage PaP1: establishment of genus PaP1-like phages.
Genomic and proteomic analyses of the terminally redundant genome of the Pseudomonas aeruginosa phage PaP1: establishment of genus PaP1-like phages.
复制标题
铜绿假单胞菌噬菌体 PaP1 末端冗余基因组的基因组和蛋白质组分析：类 PaP1 噬菌体属的建立
DOI：
10.1371/journal.pone.0062933
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hu F
中科院分区：
文献类型：
--
作者：
Lu S;Le S;Tan Y;Zhu J;Li M;Rao X;Zou L;Li S;Wang J;Jin X;Huang G;Zhang L;Zhao X;Hu F
我们分离并鉴定了一种新的铜绿假单胞菌肌病毒，命名为PaP 1。通过电子显微镜观察该噬菌体的形态，并测定其基因组序列和末端。最后，进行了基因组和蛋白质组分析。PaP 1有一个二十面体的头部，顶部直径为68-70 nm，尾部长度为138-140 nm。PaP 1基因组是一个线性双链DNA分子，含有91，715个碱基对（bp），G+C含量为49.36%，12个tRNA基因。设计了一种识别PaP 1基因组末端的策略。该基因组具有1190 bp的末端冗余。PaP 1有157个开放阅读框（ORF）。其中，143个蛋白质是已知蛋白质的同源物，但只有38个可以被功能鉴定。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和高效液相色谱-质谱法允许识别12个ORF的结构蛋白编码基因内的PaP 1基因组。比较基因组分析表明，铜绿假单胞菌噬菌体PaP 1、JG 004、PAK_P1和vB_C2_10_Ab1具有很高的相似性。除生物学特性相似外，它们还含有123个核心基因，并且具有非常接近的系统发育关系，这使它们区别于其他已知的噬菌体属。因此，我们建议，这四个细菌被归类为PaP 1样的细菌，一个新的噬菌体属的Myoviridae感染铜绿假单胞菌。
We isolated and characterized a new Pseudomonas aeruginosa myovirus named PaP1. The morphology of this phage was visualized by electron microscopy and its genome sequence and ends were determined. Finally, genomic and proteomic analyses were performed. PaP1 has an icosahedral head with an apex diameter of 68–70 nm and a contractile tail with a length of 138–140 nm. The PaP1 genome is a linear dsDNA molecule containing 91,715 base pairs (bp) with a G+C content of 49.36% and 12 tRNA genes. A strategy to identify the genome ends of PaP1 was designed. The genome has a 1190 bp terminal redundancy. PaP1 has 157 open reading frames (ORFs). Of these, 143 proteins are homologs of known proteins, but only 38 could be functionally identified. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance liquid chromatography-mass spectrometry allowed identification of 12 ORFs as structural protein coding genes within the PaP1 genome. Comparative genomic analysis indicated that the Pseudomonas aeruginosa phage PaP1, JG004, PAK_P1 and vB_PaeM_C2-10_Ab1 share great similarity. Besides their similar biological characteristics, the phages contain 123 core genes and have very close phylogenetic relationships, which distinguish them from other known phage genera. We therefore propose that these four phages be classified as PaP1-like phages, a new phage genus of Myoviridae that infects Pseudomonas aeruginosa.
登录
查看更多内容
影响因子：
4.2
作者：
Garbe J;Bunk B;Rohde M;Schobert M
通讯作者：
Schobert M
影响因子：
6.4
作者：
Alemayehu D;Casey PG;McAuliffe O;Guinane CM;Martin JG;Shanahan F;Coffey A;Ross RP;Hill C
通讯作者：
Hill C
影响因子：
14.9
作者：
Besemer, J;Borodovsky, M
通讯作者：
Borodovsky, M
影响因子：
3.7
作者：
Ceyssens, Pieter-Jan;Hertveldt, Kirsten;Lavigne, Rob
通讯作者：
Lavigne, Rob
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD