Effects of Spo0A on Clostridium acetobutylicum with an emphasis on biofilm formation
Effects of Spo0A on Clostridium acetobutylicum with an emphasis on biofilm formation
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Spo0A 对丙酮丁醇梭菌的影响，重点是生物膜形成
DOI：
10.1007/s11274-020-02859-6
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发表时间：
2020-05
影响因子：
4.1
通讯作者：
Wang Yibing
中科院分区：
文献类型：
--
作者：
Yang Zhengjiao;Wang Zhenyu;Lei Ming;Zhu Jie;Yang Yuhan;Wu Shishi;Yu Bin;Niu Huanqing;Ying Hanjie;Liu Dong;Wang Yibing
丙酮丁醇梭菌是用于生物燃料生产的众所周知的菌株。在先前的工作中，发现该菌株在发酵过程中容易形成生物膜。在我们以前的工作中，生物膜的形成可以保护细胞和提高生产力的环境压力。为探讨生物被膜形成的分子机制，对C.以丙酮丁醇为研究对象，考察了其对生物膜形成及其他生理特性的影响。当spo0A基因被破坏时，spo0A突变体几乎不能形成生物膜。spo0A突变体的聚集和粘附能力以及其群集运动性与野生型菌株相比显着降低。孢子形成也受到spo0A破坏的负面影响，在spo0A突变体发酵中几乎检测不到溶剂的产生。此外，野生型菌株和spo0A突变体之间的蛋白质组差异与生理表现一致。这是第一项确认C基因线索的研究。为进一步利用基因工程优化生物膜提供了理论依据。
Clostridium acetobutylicum is a well-known strain for biofuel production. In previous work, it was found that this strain formed biofilm readily during fermentation processes. Biofilm formation could protect cells and enhance productivities under environmental stresses in our previous work. To explore the molecular mechanism of biofilm formation, Spo0A of C. acetobutylicum was selected to investigate its influences on biofilm formation and other physiological performances. When spo0A gene was disrupted, the spo0A mutant could hardly form biofilm. The aggregation and adhesion abilities of the spo0A mutant as well as its swarming motility were dramatically reduced compared to those of wild type strain. Sporulation was also negatively influenced by spo0A disruption, and solvent production was almost undetectable in the spo0A mutant fermentation. Furthermore, proteomic differences between wild type strain and the spo0A mutant were consistent with physiological performances. This is the first study confirming a genetic clue to C. acetobutylicum biofilm and will be valuable for biofilm optimization through genetic engineering in the future.
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影响因子：
4.6
作者：
Schauer O;Mostaghaci B;Colin R;Hürtgen D;Kraus D;Sitti M;Sourjik V
通讯作者：
Sourjik V
影响因子：
3.6
作者：
Pratt, LA;Kolter, R
通讯作者：
Kolter, R
丙酮丁醇梭菌生物膜和浮游细胞的比较转录组分析
影响因子：
4.1
作者：
Liu, Dong;Xu, Jiahui;Ying, Hanjie
通讯作者：
Ying, Hanjie
DOI：
10.1093/ndt/gfl170
发表时间：
2006-08
期刊：
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
影响因子：
--
作者：
R. Shanks;J. Sargent;R. M. Martínez;Martha L. Graber;G. O’Toole
通讯作者：
R. Shanks;J. Sargent;R. M. Martínez;Martha L. Graber;G. O’Toole
影响因子：
3.6
作者：
Hamon, MA;Lazazzera, BA
通讯作者：
Lazazzera, BA