The serum proteome of Atlantic salmon, Salmo salar, during pancreas disease (PD) following infection with salmonid alphavirus subtype 3 (SAV3).
The serum proteome of Atlantic salmon, Salmo salar, during pancreas disease (PD) following infection with salmonid alphavirus subtype 3 (SAV3).
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DOI：
10.1016/j.jprot.2013.10.016
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发表时间：
2013-12-06
影响因子：
3.3
通讯作者：
Eckersall PD
中科院分区：
文献类型：
--
作者：
Braceland M;Bickerdike R;Tinsley J;Cockerill D;Mcloughlin MF;Graham DA;Burchmore RJ;Weir W;Wallace C;Eckersall PD
鲑科甲病毒是海洋大西洋鲑鱼、萨尔莫和虹鳟鱼胰腺疾病（PD）的病原体，挪威的大多数暴发由SAV亚型3（SAV 3）引起。这种非典型甲病毒是水平传播的，对水产养殖业造成重大经济影响。本组织病理学和蛋白质组学研究，使用一个建立的同居实验模型，研究组织损伤之间的相关性，在PD和一些血清蛋白与这些病理在大西洋鲑鱼。通过双向电泳、胰蛋白酶消化和肽段MS/MS指纹图谱对蛋白质进行鉴定。还鉴定了一些免疫的体液组分，其可作为该疾病的生物标志物。例如，肌酸激酶、烯醇化酶和苹果酸脱氢酶血清浓度显示与PD期间的病理学相关。相反，血红素结合蛋白，转铁蛋白和载脂蛋白，除其他外，在疾病的后期阶段改变，并没有与组织病理学。这种方法不仅对PD而且对整个鱼病都有了新的认识，通过表征蛋白质对感染的反应，通过病理过程到组织恢复。鲑科甲病毒引起大西洋鲑鱼（萨尔莫salar）的胰腺疾病（PD），并且对水产养殖业具有重大的经济影响。蛋白质组学研究的变化，以血清蛋白质组在PD已建立的实验模型的疾病。通过双向电泳、胰蛋白酶消化和肽MS/MS指纹图谱鉴定了血清蛋白质，其中72个蛋白质点在感染的12周内发生了显着变化。血清中某些蛋白质（如肌酸激酶、烯醇化酶和苹果酸脱氢酶）的浓度与组织病理学相关，而其他蛋白质（如血红素结合蛋白、转铁蛋白和载脂蛋白）在疾病后期浓度发生变化，与组织病理学无关。对感染的蛋白质反应可用于监测疾病进展并增强对PD病理学的理解。鲑鱼α病毒感染的病毒学、组织学和蛋白质组学研究用双向电泳法测定了血清蛋白质组对感染的反应，72个蛋白质点在感染过程中显示出显著的变化。使用一般线性模型，某些血清蛋白变化与组织病理学相关其他血清蛋白变化与感染反应相关
Salmonid alphavirus is the aetological agent of pancreas disease (PD) in marine Atlantic salmon, Salmo salar, and rainbow trout, Oncorhynchus mykiss, with most outbreaks in Norway caused by SAV subtype 3 (SAV3). This atypical alphavirus is transmitted horizontally causing a significant economic impact on the aquaculture industry. This histopathological and proteomic study, using an established cohabitational experimental model, investigated the correlation between tissue damage during PD and a number of serum proteins associated with these pathologies in Atlantic salmon. The proteins were identified by two-dimensional electrophoresis, trypsin digest and peptide MS/MS fingerprinting. A number of humoral components of immunity which may act as biomarkers of the disease were also identified. For example, creatine kinase, enolase and malate dehydrogenase serum concentrations were shown to correlate with pathology during PD. In contrast, hemopexin, transferrin, and apolipoprotein, amongst others, altered during later stages of the disease and did not correlate with tissue pathologies. This approach has given new insight into not only PD but also fish disease as a whole, by characterisation of the protein response to infection, through pathological processes to tissue recovery. Salmonid alphavirus causes pancreas disease (PD) in Atlantic salmon, Salmo salar, and has a major economic impact on the aquaculture industry. A proteomic investigation of the change to the serum proteome during PD has been made with an established experimental model of the disease. Serum proteins were identified by two-dimensional electrophoresis, trypsin digest and peptide MS/MS fingerprinting with 72 protein spots being shown to alter significantly over the 12 week period of the infection. The concentrations of certain proteins in serum such as creatine kinase, enolase and malate dehydrogenase were shown to correlate with tissue pathology while other proteins such as hemopexin, transferrin, and apolipoprotein, altered in concentration during later stages of the disease and did not correlate with tissue pathologies. The protein response to infection may be used to monitor disease progression and enhance understanding of the pathology of PD. A virological, histological and proteomic study of salmon alpha virus infection The serum proteome response to infection determined by two-dimensional electrophoresis 72 protein spots show significant change during infection. Certain serum protein changes related to tissue pathology using a general linear model Other serum protein change related to response to infection
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影响因子：
1.4
作者：
McLoughlin, M. F.;Graham, D. A.;Todd, D.
通讯作者：
Todd, D.
影响因子：
2
作者：
JOHNSTON, IA
通讯作者：
JOHNSTON, IA
影响因子：
2.9
作者：
Bayne, CJ;Gerwick, L;Yano, T
通讯作者：
Yano, T
影响因子：
4.7
作者：
Holland, MCH;Lambris, JD
通讯作者：
Lambris, JD
影响因子：
2.8
作者：
Hirayama, M;Kobiyama, A;Watabe, S
通讯作者：
Watabe, S