Sequential in vivo imaging of osteogenic stem/progenitor cells during fracture repair.
Sequential in vivo imaging of osteogenic stem/progenitor cells during fracture repair.
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DOI：
10.3791/51289
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发表时间：
2014-05-23
期刊：
影响因子：
--
通讯作者：
Scadden DT
中科院分区：
文献类型：
--
作者：
Park D;Spencer JA;Lin CP;Scadden DT
Bone turns over continuously and is highly regenerative following injury. Osteogenic stem/progenitor cells have long been hypothesized to exist, but in vivo demonstration of such cells has only recently been attained. Here, in vivo imaging techniques to investigate the role of endogenous osteogenic stem/progenitor cells (OSPCs) and their progeny in bone repair are provided. Using osteo-lineage cell tracing models and intravital imaging of induced microfractures in calvarial bone, OSPCs can be directly observed during the first few days after injury, in which critical events in the early repair process occur. Injury sites can be sequentially imaged revealing that OSPCs relocate to the injury, increase in number and differentiate into bone forming osteoblasts. These methods offer a means of investigating the role of stem cell-intrinsic and extrinsic molecular regulators for bone regeneration and repair.
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影响因子：
5.2
作者：
Grcevic, Danka;Pejda, Slavica;Matthews, Brya G.;Repic, Dario;Wang, Liping;Li, Haitao;Kronenberg, Mark S.;Jiang, Xi;Maye, Peter;Adams, Douglas J.;Rowe, David W.;Aguila, Hector L.;Kalajzic, Ivo
通讯作者：
Kalajzic, Ivo
影响因子：
6.2
作者：
FRIEDENSTEIN, AJ;CHAILAKHYAN, RK;KEILISSB.IV
通讯作者：
KEILISSB.IV
DOI：
10.1016/j.ecl.2005.07.009
发表时间：
2005-12-01
影响因子：
4.5
作者：
Khosla, S;Riggs, BL
通讯作者：
Riggs, BL
影响因子：
--
作者：
Tu, Qisheng;Valverde, Paloma;Chen, Jake
通讯作者：
Chen, Jake
影响因子：
64.8
作者：
Lo Celso, Cristina;Fleming, Heather E.;Wu, Juwell W.;Zhao, Cher X.;Miake-Lye, Sam;Fujisaki, Joji;Cote, Daniel;Rowe, David W.;Lin, Charles P.;Scadden, David T.
通讯作者：
Scadden, David T.