Sequence variants of the Phytophthora sojae RXLR effector Avr3a/5 are differentially recognized by Rps3a and Rps5 in soybean.
Sequence variants of the Phytophthora sojae RXLR effector Avr3a/5 are differentially recognized by Rps3a and Rps5 in soybean.
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大豆疫霉 RXLR 效应子 Avr3a/5 的序列变异体被 Rps3a 和 Rps5 差异识别
DOI：
10.1371/journal.pone.0020172
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Gijzen M
中科院分区：
文献类型：
--
作者：
Dong S;Yu D;Cui L;Qutob D;Tedman-Jones J;Kale SD;Tyler BM;Wang Y;Gijzen M
大豆疫霉无毒基因产物被相应的大豆抗性基因产物感知，引起效应子触发的免疫。过去的研究表明，大豆疫霉的Avr 3a和Avr 5基因是遗传连锁的，并且最近鉴定了编码分泌型RXLR效应蛋白的Avr 3a基因。我们现在提供的证据表明，Avr 3a和Avr 5是等位基因。来自F2后代的遗传作图数据表明Avr 3a和Avr 5共分离，并且大豆疫霉菌株集合的单倍型分析揭示了与编码Avr 3a/5的单个遗传位点一致的序列和转录多态性。进行大豆疫霉的转化和在大豆中的瞬时表达以测试Avr 3a/5等位基因如何与大豆Rps 3a和Rps 5相互作用。Avr 3a/5在通常对Rps 3a和Rps 5有毒力的大豆疫霉菌株中的过表达导致对Rps 3a和Rps 5无毒力;而Avr 3a/5的沉默导致通常对Rps 3a和Rps 5大豆品系无毒力的大豆疫霉菌株中的毒力增加。瞬时表达和与报告基因的共轰击证实了Avr 3a/5以适当的等位基因特异性方式触发Rps 5大豆叶片中的细胞死亡。Avr 3a/5基因的序列分析鉴定了效应域中区分Rps 3a和Rps 5识别的关键残基。
The perception of Phytophthora sojae avirulence (Avr) gene products by corresponding soybean resistance (Rps) gene products causes effector triggered immunity. Past studies have shown that the Avr3a and Avr5 genes of P. sojae are genetically linked, and the Avr3a gene encoding a secreted RXLR effector protein was recently identified. We now provide evidence that Avr3a and Avr5 are allelic. Genetic mapping data from F2 progeny indicates that Avr3a and Avr5 co-segregate, and haplotype analysis of P. sojae strain collections reveal sequence and transcriptional polymorphisms that are consistent with a single genetic locus encoding Avr3a/5. Transformation of P. sojae and transient expression in soybean were performed to test how Avr3a/5 alleles interact with soybean Rps3a and Rps5. Over-expression of Avr3a/5 in a P. sojae strain that is normally virulent on Rps3a and Rps5 results in avirulence to Rps3a and Rps5; whereas silencing of Avr3a/5 causes gain of virulence in a P. sojae strain that is normally avirulent on Rps3a and Rps5 soybean lines. Transient expression and co-bombardment with a reporter gene confirms that Avr3a/5 triggers cell death in Rps5 soybean leaves in an appropriate allele-specific manner. Sequence analysis of the Avr3a/5 gene identifies crucial residues in the effector domain that distinguish recognition by Rps3a and Rps5.
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影响因子：
3.7
作者：
Dong S;Qutob D;Tedman-Jones J;Kuflu K;Wang Y;Tyler BM;Gijzen M
通讯作者：
Gijzen M
DOI：
10.1073/pnas.0709303105
发表时间：
2008-03-25
影响因子：
11.1
作者：
Jiang, Rays H. Y.;Tripathy, Sucheta;Tyler, Brett M.
通讯作者：
Tyler, Brett M.
影响因子：
11.6
作者：
Dou, Daolong;Kale, Shiv D.;Tyler, Brett M.
通讯作者：
Tyler, Brett M.
影响因子：
3.7
作者：
Qutob D;Tedman-Jones J;Dong S;Kuflu K;Pham H;Wang Y;Dou D;Kale SD;Arredondo FD;Tyler BM;Gijzen M
通讯作者：
Gijzen M
影响因子：
56.9
作者：
Allen, RL;Bittner-Eddy, PD;Beynon, JL
通讯作者：
Beynon, JL