Investigation of miR396 and growth-regulating factor regulatory network in maize grain filling
Investigation of miR396 and growth-regulating factor regulatory network in maize grain filling
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玉米籽粒灌浆过程中miR396及生长调节因子调控网络的研究
DOI：
10.1007/s11738-014-1767-6
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发表时间：
2015-01
影响因子：
2.6
通讯作者：
Niu Jianxin
中科院分区：
文献类型：
--
作者：
Zhang Ke;Shi Xia;Zhao Xiaofeng;Ding Dong;Tang Jihua;Niu Jianxin
miR 396和生长调节因子（GRF）调控网络在植物物种中是进化保守的。水稻中的GRF基因与籽粒发育有关，miR 396是其靶基因。然而，关于miR 396和GRF基因参与玉米籽粒的更多信息仍然没有。本文通过对玉米籽粒发育过程中miR 396和GRF基因的RNA测序，分析其在玉米籽粒有效灌浆过程中的动态变化，并探讨其可能的调控作用。我们的结果表明，根据计算预测和degradome测序验证，GRF基因也是玉米miR 396的保守靶基因。MiR 396的表达量较高，但随着玉米籽粒灌浆的进行而逐渐下降，与其靶基因GRF基因呈显著负相关，且大多持续增加。本研究还通过实时荧光定量PCR技术验证了miR 396-GRF网络在玉米胚和胚乳发育过程中的不同调控。进一步分析表明，miR 319、miR 166和RDR在玉米籽粒发育过程中可能与miR 396-GRF网络相互作用。本研究为今后miR 396-GRF网络的综合分析奠定了基础，miR 396和GRF基因在玉米灌浆过程中的调控作用有待进一步研究。
The miR396 and growth-regulating factor (GRF) regulatory network is evolutionarily conserved among plant species. The orthologousGRFgenes in rice were involved in regulating grain development, and targeted by miR396. However, there are still no more information about the involvement of miR396 andGRFgenes in maize grain. In this article, RNA sequencing was performed on the developing maize kernels to analyze the dynamic profiles of miR396 andGRFgenes and explore their possible regulatory roles during maize effective grain filling. Our results showed thatGRFgenes were also the conserved targets of miR396 in maize, according to computational prediction and validated by degradome sequencing. MiR396 expressed high and gradually declined with advancing maize grain filling, significant negatively correlated with its targetGRFgenes, which mostly increased continuously. Our test also provided a testimony of miR396-GRF network in different regulations between the development of maize embryo and endosperm by real-time quantitative PCR. And further analysis of expression pattern suggested that miR319, miR166, and RDR might interact with miR396-GRF network during grain development in maize. This study mainly provided a valuable foundation for future comprehensive analysis of miR396-GRF network, and further research work is needed to confirm the regulatory roles of miR396 andGRFgenes during grain filling in maize.
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