MicroRNA transcriptomic analysis of heterosis during maize seed germination.
MicroRNA transcriptomic analysis of heterosis during maize seed germination.
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玉米种子萌发过程杂种优势的 MicroRNA 转录组分析
DOI：
10.1371/journal.pone.0039578
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tang J
中科院分区：
文献类型：
--
作者：
Ding D;Wang Y;Han M;Fu Z;Li W;Liu Z;Hu Y;Tang J
杂种优势在玉米等作物育种中得到了广泛的应用，在提高产量、改善品质、增强抗逆性等方面发挥着重要作用，但杂种优势产生的分子机理还不清楚。为了阐明miRNA依赖的基因调控是否是玉米萌发过程中杂种优势的原因，将深度测序技术应用于在中国广泛种植的玉米杂交种豫玉22及其亲本自交系豫87 -1和宗3的萌发胚。利用实时荧光定量PCR技术，对杂交株系和亲本株系中显著表达的几种miRNAs的靶基因进行了预测和检测。共检测到107个玉米miRNAs保守序列。这些miRNAs中的大多数在杂交体中与其亲本系相比以非加性方式表达。这些结果表明，miRNAs可能参与了玉米萌发过程中的杂种优势形成，并通过其靶基因的降解发挥作用。根据严格的标准预测新的miRNA，并且只有在所有三个样品中检测到的miRNA被视为新的玉米miRNA。在萌发的玉米种子中，总共预测了属于20个miRNA家族的34个miRNA。在杂种中，miRNAs的全面抑制可能导致基因表达增强，这可能是杂种与其亲本系相比表现出更高的胚萌发活力的原因之一。
Heterosis has been utilized widely in the breeding of maize and other crops, and plays an important role in increasing yield, improving quality and enhancing stresses resistance, but the molecular mechanism responsible for heterosis is far from clear. To illustrate whether miRNA-dependent gene regulation is responsible for heterosis during maize germination, a deep-sequencing technique was applied to germinating embryos of a maize hybrid, Yuyu22, which is cultivated widely in China and its parental inbred lines, Yu87-1 and Zong3. The target genes of several miRNAs showing significant expression in the hybrid and parental lines were predicted and tested using real-time PCR. A total of 107 conserved maize miRNAs were co-detected in the hybrid and parental lines. Most of these miRNAs were expressed non-additively in the hybrid compared to its parental lines. These results indicated that miRNAs might participate in heterosis during maize germination and exert an influence via the decay of their target genes. Novel miRNAs were predicted follow a rigorous criterion and only the miRNAs detected in all three samples were treated as a novel maize miRNA. In total, 34 miRNAs belonged to 20 miRNA families were predicted in germinating maize seeds. Global repression of miRNAs in the hybrid, which might result in enhanced gene expression, might be one reason why the hybrid showed higher embryo germination vigor compared to its parental lines.
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