Characterization of gene expression associated with drought avoidance and tolerance traits in a perennial grass species.
Characterization of gene expression associated with drought avoidance and tolerance traits in a perennial grass species.
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多年生禾本科植物抗旱和耐旱性状相关基因表达的表征
DOI：
10.1371/journal.pone.0103611
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Huang B
中科院分区：
文献类型：
--
作者：
Zhou P;An Y;Wang Z;Du H;Huang B
为了解多年生牧草适应干旱胁迫的分子机制，本研究对C4杂交狗牙根[Cynodon dactgon(L.)Pers.×横纹盘藻Burtt Davy，cv.Tifway]和普通狗牙根(C.dactylon，cv.C299)。耐旱‘Tifway’和干旱敏感‘C299’的植株在生长室内不灌水，分别干旱5d(轻度胁迫)和10d(重度胁迫)。在干旱胁迫第5天和第10天，‘Tifway’的电解质渗透率显著低于‘C299’，而相对含水量则显著高于‘C299’。利用抑制性消减杂交技术构建了4个抗旱基因文库，共鉴定出干旱胁迫第5天和第10天两个基因型的277个干旱响应基因，主要分为胁迫防御、代谢、渗透调节、膜系统、信号与调节、结构蛋白、蛋白质合成与降解、能量代谢等功能类别。定量-PCR分析证实了36个干旱上调基因在耐旱‘Tifway’中的表达高于干旱敏感的‘C299’，其中包括与干旱避免性状(如角质蜡形成(CER1和甾醇去饱和酶))、耐旱性状(如脱水保护蛋白(脱水蛋白、HVA-22类似蛋白)和氧化胁迫防御(超氧化物歧化酶、脱氢抗坏血酸还原酶、2-半胱氨酸过氧化还原酶)和胁迫信号(EREBP-4类蛋白和WRKY转录因子)的表达。这些结果表明，胁迫信号、角质层蜡质积累、抗氧化防御和脱水保护蛋白积累等基因的表达对于暖季多年生牧草适应长期干旱胁迫可能是至关重要的。
To understand molecular mechanisms of perennial grass adaptation to drought stress, genes associated with drought avoidance or tolerance traits were identified and their expression patterns were characterized in C4 hybrid bermudagrass [Cynodon dactylon (L.) Pers.×C. transvaalensis Burtt Davy, cv. Tifway] and common bermudagrass (C. dactylon, cv. C299). Plants of drought-tolerant ‘Tifway’ and drought-sensitive ‘C299’ were exposed to drought for 5 d (mild stress) and 10 d (severe stress) by withholding irrigation in a growth chamber. ‘Tifway’ maintained significantly lower electrolyte leakage and higher relative water content than ‘C299’ at both 5 and 10 d of drought stress. Four cDNA libraries via suppression subtractive hybridization analysis were constructed and identified 277 drought-responsive genes in the two genotypes at 5 and 10 d of drought stress, which were mainly classified into the functional categories of stress defense, metabolism, osmoregulation, membrane system, signal and regulator, structural protein, protein synthesis and degradation, and energy metabolism. Quantitative-PCR analysis confirmed the expression of 36 drought up-regulated genes that were more highly expressed in drought-tolerant ‘Tifway’ than drought-sensitive ‘C299’, including those for drought avoidance traits, such as cuticle wax formation (CER1 and sterol desaturase), for drought tolerance traits, such as dehydration-protective proteins (dehydrins, HVA-22-like protein) and oxidative stress defense (superoxide dismutase, dehydroascorbate reductase, 2-Cys peroxiredoxins), and for stress signaling (EREBP-4 like protein and WRKY transcription factor). The results suggest that the expression of genes for stress signaling, cuticle wax accumulation, antioxidant defense, and dehydration-protective protein accumulation could be critically important for warm-season perennial grass adaptation to long-term drought stress.
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