Molecular characterization of US-like and Asian non-S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013-2016 and PEDVs collected from recurrent outbreaks.
Molecular characterization of US-like and Asian non-S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013-2016 and PEDVs collected from recurrent outbreaks.
复制标题
DOI：
10.1186/s12917-018-1409-0
复制
发表时间：
2018-03-14
影响因子：
2.6
通讯作者：
Yamaguchi R
中科院分区：
文献类型：
--
作者：
Van Diep N;Sueyoshi M;Norimine J;Hirai T;Myint O;Teh APP;Izzati UZ;Fuke N;Yamaguchi R
自2013年底以来，猪流行性腹泻病毒（PEDV）在日本再次出现，给养猪业造成了严重的经济损失。虽然PEDV疫苗已被广泛使用，但该病已迅速席卷全国，在接种PED疫苗的农场中常见，并在国内牛群中复发。为了更好地了解导致日本再次爆发的PEDV，对2013-2016年期间在日本收集的45种PEDV的全长刺突（S），膜（M）和核衣壳（N）基因进行了测序和分析。基于S基因序列的系统发育分析显示，所有最近的现场PEDV在遗传上不同于经典的日本株，并被分为三种基因型：北美（NA），S INDEL，和亚洲非S INDEL。我们的数据表明，多个亲本PEDV毒株可能是在同一时间或相似时间从国外引入日本的。新发现的日本菌株与美国菌株的关系最密切。在日本流行的日本菌株的两个亚系与在美国鉴定的两个亚系相似，表明这些菌株有共同的祖先。与日本使用的两种疫苗株相比，野毒株在表位区域、糖基化位点和磷酸化位点上发生了各种变化。这些取代，特别是在S（521、553、568和570）、M（5）和N（123、252和255）蛋白的表位区域中观察到的取代，可能影响抗原性和疫苗效力，导致不成功的PEDV控制。从三个畜群的原发性和继发性暴发中收集的PEDV之间的序列比较显示，该疾病已经发展到地方流行阶段，其中PEDV可以在畜群或局部地区持续近两年，引起随后的流行。这些结果阐明了在日本传播的PEDV的遗传特征、起源和分子流行病学，以及引起反复爆发的PEDV毒株。这项研究提供了一个更好的了解PEDV负责最近在日本爆发，并可能有助于制定控制和预防疾病的措施。本文的在线版本（10.1186/s12917-018-1409-0）包含补充材料，可供授权用户使用。
Since late 2013, porcine epidemic diarrhea virus (PEDV) has reemerged in Japan and caused severe economic losses to the swine industry. Although PEDV vaccines have been used widely, the disease has swept rapidly across the county, and is commonly observed in PED-vaccinated farms, and has recurred in domestic herds. To better understand PEDVs responsible for the reemerging outbreaks in Japan, full-length spike (S), membrane (M), and nucleocapsid (N) genes of 45 PEDVs collected in Japan during 2013–2016, were sequenced and analyzed. Phylogenetic analysis based on S gene sequences revealed that all the recent field PEDVs were genetically distinct from the classical Japanese strains, and were classified into three genotypes: North American (NA), S INDEL, and Asian non-S INDEL. Our data suggested a possibility that multiple parental PEDV strains were introduced into Japan from abroad at the same time or similar times. The newly identified Japanese strains showed the closest relationship to the US strains. Two sublineages of Japanese strains circulating in Japan were similar to two sublineages identified in the US, suggesting common ancestors for these strains. In comparison with two vaccine strains used in Japan, the field strains had various changes in epitope regions, glycosylation sites, and phosphorylation sites. These substitutions, particularly observed in epitope regions of the S (521, 553, 568, and 570), M (5), and N (123, 252, and 255) proteins, may have affected antigenicity and vaccine efficacy, resulting in an unsuccessful PEDV control. Sequence comparisons between PEDVs collected from primary and secondary outbreaks in three herds revealed that the disease has developed to an endemic stage in which PEDV could persist for nearly two years in the herds or local regions, causing subsequent epidemics. These results elucidate the genetic characteristics, origin, and molecular epidemiology of PEDVs circulating in Japan, as well as the PEDV strains causing recurrent outbreaks. This study provides a better insight into the PEDVs responsible for recent outbreaks in Japan, and could potentially help to develop measures for controlling and preventing the disease. The online version of this article (10.1186/s12917-018-1409-0) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
4.8
作者：
Lee C
通讯作者：
Lee C
中国猪流行性腹泻病毒刺突基因检测及分子多样性
DOI：
10.3390/v5102601
发表时间：
2013-10-22
期刊：
Viruses
影响因子：
--
作者：
Chen J;Liu X;Shi D;Shi H;Zhang X;Li C;Chi Y;Feng L
通讯作者：
Feng L
影响因子：
5
作者：
Cruz DJ;Kim CJ;Shin HJ
通讯作者：
Shin HJ
影响因子：
3.7
作者：
Spencer KA;Dee M;Britton P;Hiscox JA
通讯作者：
Hiscox JA
影响因子：
6.4
作者：
Huang YW;Dickerman AW;Piñeyro P;Li L;Fang L;Kiehne R;Opriessnig T;Meng XJ
通讯作者：
Meng XJ