Genome-wide investigation and expression analysis suggest diverse roles and genetic redundancy of Pht1 family genes in response to Pi deficiency in tomato.
Genome-wide investigation and expression analysis suggest diverse roles and genetic redundancy of Pht1 family genes in response to Pi deficiency in tomato.
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全基因组研究和表达分析表明 Pht1 家族基因在番茄 Pi 缺乏中的不同作用和遗传冗余
DOI：
10.1186/1471-2229-14-61
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发表时间：
2014-03-11
影响因子：
5.3
通讯作者：
Xu G
中科院分区：
文献类型：
--
作者：
Chen A;Chen X;Wang H;Liao D;Gu M;Qu H;Sun S;Xu G
背景缺磷是限制植物生长的主要营养胁迫之一。植物对磷的吸收被认为是由一些属于Pht 1家族的高亲和力磷酸盐（Pi）转运蛋白介导的。虽然Pht 1基因已被广泛确定在几种植物中，有一个缺乏系统的分析的Pht 1基因家族在任何茄科species thereof.ResultsHere，我们报告的全基因组分析，系统发育进化和表达模式的Pht 1基因在番茄（番茄）。通过对番茄基因组序列数据库的广泛检索，共鉴定出8个Pht 1基因（LePT 1 to 8），分布于3条染色体（3，6，9）上。染色体组构和系统发育树分析表明，这6个Pht 1旁系同源物LePT 1/3、LePT 2/6和LePT 4/5是在茄科与双子叶植物其他科分离后发生的串联重复事件中产生的。对这些Pht 1成员的表达分析表明，除LePT 8在所有组织中均未检测到其转录本外，其余7个Pht 1旁系同源基因均表现出差异但部分重叠的表达模式，其中LePT 1和LePT 7在所有组织中普遍表达，且其转录本在磷饥饿反应中被大量诱导; LePT 2和LePT 6是两个具有相同编码序列的旁系同源基因，主要在缺Pi的根中表达; LePT 3、LePT 4和LePT 5在低磷条件下被丛枝菌根真菌侵染的根系中被强烈激活，而在高磷条件下没有被激活。组织化学分析表明，含有MYCS和P1 BS两个元件的1250-bpLePT 3启动子片段和471-bpLePT 5启动子片段足以指导GUS报告基因在菌根根中的表达，并且仅限于具有AM真菌结构的不同细胞。此外，4个旁系同源物，LePT 1，LePT 2，LePT 6和LePT 7，非常显着下调，在菌根根在低磷供应condition.ConclusionsThe研究结果提供了新的见解Pht 1基因的进化扩展，功能分歧和遗传冗余，在番茄缺磷和菌根共生。
BackgroundPhosphorus (P) deficiency is one of the major nutrient stresses limiting plant growth. The uptake of P by plants is well considered to be mediated by a number of high-affinity phosphate (Pi) transporters belonging to the Pht1 family. Although the Pht1 genes have been extensively identified in several plant species, there is a lack of systematic analysis of the Pht1 gene family in any solanaceous species thus far.ResultsHere, we report the genome-wide analysis, phylogenetic evolution and expression patterns of the Pht1 genes in tomato (Solanum lycopersicum). A total of eight putative Pht1 genes (LePT1to8), distributed on three chromosomes (3, 6 and 9), were identified through extensive searches of the released tomato genome sequence database. Chromosomal organization and phylogenetic tree analysis suggested that the six Pht1 paralogues,LePT1/3,LePT2/6 andLePT4/5, which were assigned into three pairs with very close physical distance, were produced from recent tandem duplication events that occurred after Solanaceae splitting with other dicot families. Expression analysis of these Pht1 members revealed that exceptLePT8, of which the transcript was undetectable in all tissues, the other seven paralogues showed differential but partial-overlapping expression patterns.LePT1andLePT7were ubiquitously expressed in all tissues examined, and their transcripts were induced abundantly in response to Pi starvation;LePT2andLePT6, the two paralogues harboring identical coding sequence, were predominantly expressed in Pi-deficient roots;LePT3,LePT4andLePT5were strongly activated in the roots colonized by arbuscular mycorrhizal fungi under low-P, but not high-P condition. Histochemical analysis revealed that a 1250-bpLePT3promoter fragment and a 471-bpLePT5promoter fragment containing the two elements, MYCS and P1BS, were sufficient to direct the GUS reporter expression in mycorrhizal roots and were limited to distinct cells harboring AM fungal structures. Additionally, the four paralogues,LePT1,LePT2,LePT6andLePT7, were very significantly down-regulated in the mycorrhizal roots under low Pi supply condition.ConclusionsThe results obtained from this study provide new insights into the evolutionary expansion, functional divergence and genetic redundancy of the Pht1 genes in response to Pi deficiency and mycorrhizal symbiosis in tomato.
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