Differential assembly of root-associated bacterial and fungal communities of a dual transgenic insect-resistant maize line at different host niches and different growth stages.
Differential assembly of root-associated bacterial and fungal communities of a dual transgenic insect-resistant maize line at different host niches and different growth stages.
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双转基因抗虫玉米品系在不同寄主生态位和不同生长阶段根部相关细菌和真菌群落的差异组装
DOI：
10.3389/fmicb.2022.1023971
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发表时间：
2022
影响因子：
5.2
通讯作者：
Yang, Yonghua
中科院分区：
文献类型：
--
作者：
Wen, Zhongling;Yao, Weixuan;Han, Mi;Xu, Xinhong;Wu, Fengci;Yang, Minkai;Fazal, Aliya;Yin, Tongming;Qi, Jinliang;Lu, Guihua;Yang, Rongwu;Song, Xinyuan;Yang, Yonghua
转基因技术已广泛应用于农作物开发，转基因玉米是世界第二大转基因作物。尽管根际细菌和真菌种群是植物性能的关键调节因子，但很少有研究评估转基因玉米对这些群落的影响。本研究中使用的植物材料包括对照玉米品系 B73 和 mcry1Ab 和 mcry2Ab 双转基因抗虫玉米品系 2A-7。植物和土壤样品在三个生长阶段（拔节期、开花期和成熟期）采样，采样区从根部外部到内部分别为周围土壤（SS）、根际土壤（RS）和完整根（RT）。本研究中α多样性的结果显示，从根的外部到内部，群落丰富度和多样性下降，而群落覆盖度增加。此外，根据统计分析，玉米根际和玉米发育阶段的不同寄主生态位影响β多样性。与 B73 相比，转基因玉米品系 2A-7 对微生物群落的组成没有显着影响。与RS和SS相比，宿主生态位RT倾向于在门水平上消耗Chloroflexi、Gemmatimonadetes和Mortierellomycota。与RS和SS相比，在RT生态位中固氮细菌Pseudomonas、Herbaspirillum huttiense、Rhizobium leguminosarum和Sphingomonas azotifigens富集，而与拔节和开花阶段相比，在成熟阶段芽孢杆菌增多，寡养单胞菌减少。固氮蛋白 FixH（直系同源群簇，COG5456）被发现在 RT 中含量丰富。此外，还发现引起玉米茎腐病的病原真菌根全全酵母（Gaeumannomyces radicicola）在RT中含量丰富，而有益真菌透明被孢霉（Mortierella hyalina）在RT中含量减少。最后，G. radicicola 的丰度在玉米的发育过程中逐渐增加。总之，转基因玉米中根部相关微生物群落的差异组装主要是由整个土壤-植物连续体中的宿主生态位而非Bt抗虫基因或Bt蛋白分泌负责，这为生态农业提供了理论基础。
Transgenic technology has been widely applied to crop development, with genetically modified (GM) maize being the world’s second-largest GM crop. Despite the fact that rhizosphere bacterial and fungal populations are critical regulators of plant performance, few studies have evaluated the influence of GM maize on these communities. Plant materials used in this study included the control maize line B73 and the mcry1Ab and mcry2Ab dual transgenic insect-resistant maize line 2A-7. The plants and soils samples were sampled at three growth stages (jointing, flowering, and maturing stages), and the sampling compartments from the outside to the inside of the root are surrounding soil (SS), rhizospheric soil (RS), and intact root (RT), respectively. In this study, the results of alpha diversity revealed that from the outside to the inside of the root, the community richness and diversity declined while community coverage increased. Morever, the different host niches of maize rhizosphere and maize development stages influenced beta diversity according to statistical analysis. The GM maize line 2A-7 had no significant influence on the composition of microbial communities when compared to B73. Compared to RS and SS, the host niche RT tended to deplete Chloroflexi, Gemmatimonadetes and Mortierellomycota at phylum level. Nitrogen-fixation bacteria Pseudomonas, Herbaspirillum huttiense, Rhizobium leguminosarum, and Sphingomonas azotifigens were found to be enriched in the niche RT in comparison to RS and SS, whilst Bacillus was found to be increased and Stenotrophomonas was found to be decreased at the maturing stage as compared to jointing and flowering stages. The nitrogen fixation protein FixH (clusters of orthologous groups, COG5456), was found to be abundant in RT. Furthermore, the pathogen fungus that causes maize stalk rot, Gaeumannomyces radicicola, was found to be abundant in RT, while the beneficial fungus Mortierella hyalina was found to be depleted in RT. Lastly, the abundance of G. radicicola gradually increased during the development of maize. In conclusion, the host niches throughout the soil-plant continuum rather than the Bt insect-resistant gene or Bt protein secretion were primarily responsible for the differential assembly of root-associated microbial communities in GM maize, which provides the theoretical basis for ecological agriculture.
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