Ss-Sl2, a novel cell wall protein with PAN modules, is essential for sclerotial development and cellular integrity of Sclerotinia sclerotiorum.
Ss-Sl2, a novel cell wall protein with PAN modules, is essential for sclerotial development and cellular integrity of Sclerotinia sclerotiorum.
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Ss-Sl2 是一种具有 PAN 模块的新型细胞壁蛋白，对于核盘菌的菌核发育和细胞完整性至关重要
DOI：
10.1371/journal.pone.0034962
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fu Y
中科院分区：
文献类型：
--
作者：
Yu Y;Jiang D;Xie J;Cheng J;Li G;Yi X;Fu Y
菌核是许多植物真菌病原体的重要休眠体。在这里，我们报道了一种名为 Ss-Sl2 的蛋白质参与核盘菌的菌核发育。 Ss-Sl2 与任何已知功能的蛋白质没有表现出显着的同源性。 Ss-Sl2 包含两个假定的 PAN 模块，这些模块在具有不同粘附功能的其他蛋白质中被发现。 Ss-Sl2是一种分泌蛋白，在菌核发育初期，分泌大量的Ss-Sl2并积累在细胞壁上。 RNAi介导的Ss-Sl2沉默菌株维持细胞完整性的能力降低，但Ss-Sl2沉默菌株的菌丝生长和毒力与野生菌株没有显着差异。 Ss-Sl2沉默菌株在菌核发育初期可形成交织的菌丝团，但交织的菌丝不能固结和黑化。这些交织体中的菌丝壁薄，排列松散。免疫共沉淀和酵母双杂交实验表明，3-磷酸甘油醛脱氢酶（GAPDH）、Woronin 体主要蛋白（Hex1）和延伸因子 1-α 与 Ss-Sl2 相互作用。 GAPDH 敲低菌株在菌核发育中表现出与 Ss-Sl2 沉默菌株相似的表型。 Hex1 敲除菌株在维持菌丝完整性方面表现出与 Ss-Sl2 沉默菌株类似的损害。结果表明，Ss-Sl2 在核盘菌的菌核发育和细胞完整性中发挥作用。
The sclerotium is an important dormant body for many plant fungal pathogens. Here, we reported that a protein, named Ss-Sl2, is involved in sclerotial development of Sclerotinia sclerotiorum. Ss-Sl2 does not show significant homology with any protein of known function. Ss-Sl2 contains two putative PAN modules which were found in other proteins with diverse adhesion functions. Ss-Sl2 is a secreted protein, during the initial stage of sclerotial development, copious amounts of Ss-Sl2 are secreted and accumulated on the cell walls. The ability to maintain the cellular integrity of RNAi-mediated Ss-Sl2 silenced strains was reduced, but the hyphal growth and virulence of Ss-Sl2 silenced strains were not significantly different from the wild strain. Ss-Sl2 silenced strains could form interwoven hyphal masses at the initial stage of sclerotial development, but the interwoven hyphae could not consolidate and melanize. Hyphae in these interwoven bodies were thin-walled, and arranged loosely. Co-immunoprecipitation and yeast two-hybrid experiments showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Woronin body major protein (Hex1) and elongation factor 1-alpha interact with Ss-Sl2. GAPDH-knockdown strains showed a similar phenotype in sclerotial development as Ss-Sl2 silenced strains. Hex1-knockdown strains showed similar impairment in maintenance of hyphal integrity as Ss-Sl2 silenced strains. The results suggested that Ss-Sl2 functions in both sclerotial development and cellular integrity of S. sclerotiorum.
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影响因子：
3
作者：
Jurick, Wayne M., II;Rollins, Jeffrey A.
通讯作者：
Rollins, Jeffrey A.
影响因子：
4.8
作者：
Brecht, S;Carruthers, VB;Soldati, D
通讯作者：
Soldati, D
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3.5
作者：
Erental, A.;Harel, A.;Yarden, O.
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影响因子：
3.5
作者：
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影响因子：
2.7
作者：
Jurick, WM;Dickman, MB;Rollins, JA
通讯作者：
Rollins, JA