Visible and near infrared autofluorescence and hyperspectral imaging spectroscopy for the investigation of colorectal lesions and detection of exogenous fluorophores
Visible and near infrared autofluorescence and hyperspectral imaging spectroscopy for the investigation of colorectal lesions and detection of exogenous fluorophores
复制标题
用于研究结直肠病变和检测外源荧光团的可见光和近红外自发荧光和高光谱成像光谱
DOI：
--
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
D. Elson
中科院分区：
文献类型：
--
作者：
Kevin R. Koh;Tobias C. Wood;R. Goldin;Guang;D. Elson
结肠镜检查是临床监测结直肠癌最常用的结肠镜检查方法。这种基于白光的成像技术通常用于检测腺瘤性息肉，腺瘤性息肉是癌症的前驱物，随后被切除以防止进展为结肠癌。然而，一些腺瘤小而平，即使是最有经验的结肠镜检查人员也很难将它们与非肿瘤组织区分开来。我们报道了一种用于执行激发分辨可见光和近红外(NIR)自体荧光成像和高光谱反射成像的光学系统，用于研究胃肠道肿瘤和非肿瘤粘膜之间的光谱特征的差异。这是使用我们的多模式腹腔镜成像系统在体外结肠组织上演示的，该系统由一个超连续激发源和一个用于可调波长选择的数字多镜装置(DMD)组成。我们还演示了使用ISOMAP降维技术对应用于组织的外源荧光团的识别，以可视化所获得的图像数据。
Colonoscopy is the most widely prescribed method of colonic imaging for the clinical surveillance of colorectal cancer. This white light-based imaging technique is often used for the detection of adenomatous polyps, the dysplastic precursors of cancer, that are subsequently removed to prevent progression to colonic carcinoma. However, some adenomas are diminutive and flat, making them difficult to discriminate from non-neoplastic tissue even for the most experienced of colonoscopists. We report here an optical system for performing excitation resolved visible and near-infrared (NIR) autofluorescence imaging and hyperspectral reflectance imaging for the investigation of differences in the spectral signatures between neoplastic and non-neoplastic mucosa along the gastrointestinal tract. This is demonstrated on ex vivo colorectal tissues using our multimodal laparoscopic imaging system, which consists of a supercontinuum excitation source and a digital multimirror device (DMD) for tuneable wavelength selection. We also demonstrate the discrimination of exogenous fluorophores applied to the tissue using the Isomap dimensionality reduction technique for visualisation of the acquired image data.
影响因子：
3.5
作者：
Sokolov, K;Galvan, J;Richards-Kortum, R
通讯作者：
Richards-Kortum, R
影响因子：
3.5
作者：
Drezek, R;Sokolov, K;Richards-Kortum, R
通讯作者：
Richards-Kortum, R
影响因子：
3.5
作者：
Roblyer, Darren;Richards-Kortum, Rebecca;Gillenwater, Ann M.
通讯作者：
Gillenwater, Ann M.