Imaging single photons and intrinsic optical signals for studies of vesicular and non-vesicular ATP release from axons.
Imaging single photons and intrinsic optical signals for studies of vesicular and non-vesicular ATP release from axons.
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DOI：
10.3389/fnana.2011.00032
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发表时间：
2011
影响因子：
2.9
通讯作者：
Fields RD
中科院分区：
文献类型：
--
作者：
Fields RD
神经递质释放的时间和空间动力学是理解轴突和其他细胞(包括神经元、胶质细胞和血管细胞)之间依赖活动的信号转导的基础。描述了一种显微成像技术，该技术能够研究神经递质ATP从轴突释放对动作电位的反应。该方法结合了单光子成像、本征光学信号成像和高倍率延时显微镜，能够研究动作电位诱导的ATP释放以及细胞形态和活动依赖的轴突肿胀。从轴突释放的三磷酸腺苷催化荧光素和荧光素酶之间的化学发光反应，产生可以单独成像的单个光子。除了囊泡释放外，通过轴突肿胀激活的膜通道的ATP释放与固有的光信号同步监测。在轴突局部15 μm区域观察到重复的光子发射，其频率分布不同于正态分布，也不同于这些局部区域外的发射频率。
The temporal and spatial dynamics of neurotransmitter release are fundamental to understanding activity-dependent signaling between axons and other cells, including neurons, glia, and vascular cells. A microscopic imaging technique is described that enables studying release of the neurotransmitter ATP from axons in response to action potentials. The method combines imaging single-photons, intrinsic optical signal imaging, and high magnification time-lapse microcopy to enable investigations of action potential-induced ATP release together with cell morphology and activity-dependent axon swelling. ATP released from axons catalyzes a chemiluminescent reaction between luciferin and luciferase that generates single photons that can be imaged individually. In addition to vesicular release, ATP release through membrane channels activated by axon swelling was monitored simultaneously with intrinsic optical signals. Repeated emissions of photons were observed from localized 15 μm regions of axons, with a frequency distribution that differed from a normal distribution and from the frequency of emissions outside these localized regions.
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影响因子：
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作者：
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通讯作者：
Ni Y
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