Induction of the expressions of antioxidant enzymes by luteinizing hormone in the bovine corpus luteum.
Induction of the expressions of antioxidant enzymes by luteinizing hormone in the bovine corpus luteum.
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DOI：
10.1262/jrd.2012-186
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Okuda K
中科院分区：
文献类型：
--
作者：
Kawaguchi S;Sakumoto R;Okuda K
黄体生成素（LH）参与维持牛黄体（CL）功能的黄体保护机制尚未完全阐明。由于抗氧化酶是许多哺乳动物CL中的抗凋亡因子，我们假设LH的黄体保护作用是通过刺激局部抗氧化酶的产生和作用来介导的。为了验证上述假设，在本研究中，我们研究了黄体生成素保护黄体作用的机制。取黄体中期（发情周期第8-12天）培养的牛黄体细胞，分别用黄体生成素（10 ng/ml）、onapristone （OP，一种特异性黄体酮受体拮抗剂，100 μM）和diethyldithiocarbamate (DETC；LH联合或不联合OP均可显著提高锰超氧化物歧化酶（Mn-SOD）和过氧化氢酶（CATA） mRNA和蛋白的表达以及SOD活性。单用LH可显著提高含铜、锌的SOD (Cu、Zn-SOD) mRNA和蛋白的表达，而OP联合或不联合LH可显著降低含铜、锌-SOD mRNA和蛋白的表达。Cu、Zn-SOD、Mn-SOD和CATA mRNA的表达在黄体中期高于其他黄体期。LH联合DETC显著降低LH-增加细胞活力。综上所述，黄体生成素通过增加黄体生成素的抗氧化酶来提高细胞活力，从而维持黄体期黄体细胞的功能。
Luteoprotective mechanisms of luteinizing hormone (LH) involved in the maintenance of bovine corpus luteum (CL) function have not been completely clarified. Since antioxidant enzymes are well documented as antiapoptotic factors in the CL of many mammals, we hypothesized that the luteoprotective action of LH is mediated by stimulating the local production and action of antioxidant enzymes. To test the above hypothesis, in the present study, we examined the mechanisms involved in the luteoprotective actions of LH. Cultured bovine luteal cells obtained from the CL at the mid-luteal stage (days 8–12 of the estrous cycle) were treated with LH (10 ng/ml), onapristone (OP; a specific progesterone receptor antagonist, 100 μM) and diethyldithiocarbamate [DETC; an inhibitor of superoxide dismutase (SOD), 100 μM] for 24 h. LH in combination with or without OP significantly increased the mRNA and protein expressions of manganese SOD (Mn-SOD) and catalase (CATA) and SOD activity. While LH alone significantly increased the mRNA and protein expressions of SOD containing copper and zinc (Cu,Zn-SOD), OP in combination with or without LH significantly decreased the mRNA and protein expressions of Cu,Zn-SOD. In addition, Cu,Zn-SOD, Mn-SOD and CATA mRNA expressions were higher at the mid luteal phase than the other luteal phases. LH in combination with DETC significantly decreased LH-increased cell viability. The overall results suggest that LH increases cell viability by LH-increased antioxidant enzymes, resulting in maintenance of CL function during the luteal phase in cattle.
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影响因子：
1.8
作者：
Korzekwa, Anna J.;Okuda, Kiyoshi;Skarzynski, Dariusz J.
通讯作者：
Skarzynski, Dariusz J.
影响因子：
3.6
作者：
PETERS, KE;BERGFELD, EG;KINDER, JE
通讯作者：
KINDER, JE
影响因子：
2.1
作者：
Sakumoto, Ryosuke;Komatsu, Tokushi;Okuda, Kiyoshi
通讯作者：
Okuda, Kiyoshi
影响因子：
3.6
作者：
Okuda, K;Uenoyama, Y;Kimura, T
通讯作者：
Kimura, T
DOI：
10.1016/s0304-4165(97)00136-0
发表时间：
1998-03-12
影响因子：
3
作者：
Rapoport, R;Sklan, D;Hanukoglu, I
通讯作者：
Hanukoglu, I