3D bioprinter applied picosecond pulsed electric fields for targeted manipulation of proliferation and lineage specific gene expression in neural stem cells.
3D bioprinter applied picosecond pulsed electric fields for targeted manipulation of proliferation and lineage specific gene expression in neural stem cells.
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DOI：
10.1088/1741-2552/aac8ec
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发表时间：
2018-10
影响因子：
4
通讯作者：
Sachs PC
中科院分区：
文献类型：
--
作者：
Petrella RA;Mollica PA;Zamponi M;Reid JA;Xiao S;Bruno RD;Sachs PC
皮秒脉冲电场（psPEF）具有以非接触方式引起哺乳动物细胞功能变化的潜力。这种对多能细胞和多能细胞的电操纵可以作为神经界面和组织工程的一种工具。在这里，我们描述了psPEF在指导神经干细胞（NSCs）基因表达、代谢和增殖方面的潜力。作为比较，间充质干细胞（MSCs）也被测试。psPEF电极固定在定制的市售3d打印机上，这使我们能够以高空间精度传递脉冲，并系统地控制电极在三轴上的位置。当电极连续通电并通过3d打印机移动其位置时，表面上的大量细胞可以暴露在均匀的psPEF下。在两种电场强度（20和40 kV/cm）下，定量分析细胞反应，包括细胞活力、增殖和基因表达测定。分析显示，NSCs和MSCs在治疗后均未出现明显的细胞死亡。两种细胞类型都表现出增加的代谢减少；而MSCs的反应率对电场强度的变化较为敏感，而NSCs的反应率与电场强度无关。增殖率的变化是细胞类型特异性的。MSCs的增殖无明显变化，而NSCs表现出电场依赖性反应，电场越高增殖越少。此外，NSCs在24小时后胶质原纤维酸性蛋白（GFAP）上调至40 kV/cm，这是星形胶质细胞特异性分化的特征。
Picosecond pulse electric fields (psPEF) have the potential to elicit functional changes in mammalian cells in a non-contact manner. Such electro-manipulation of pluripotent and multipotent cells could be a tool in both neural interface and tissue engineering. Here, we describe the potential of psPEF in directing neural stem cells (NSCs) gene expression, metabolism, and proliferation. As a comparison mesenchymal stem cells (MSCs) were also tested. A psPEF electrode was anchored on a customized commercially available 3-D printer, which allowed us to deliver pulses with high spatial precision and systematically control the electrode position in three-axes. When the electrodes are continuously energized and their position is shifted by the 3-D printer, large numbers of cells on a surface can be exposed to a uniform psPEF. With two electric field strengths (20 and 40 kV/cm), cell responses, including cell viability, proliferation, and gene expression assays, were quantified and analyzed. Analysis revealed both NSCs and MSCs showed no significant cell death after treatments. Both cell types exhibited an increased metabolic reduction; however, the response rate for MSCs was sensitive to the change of electric field strength, but for NSCs, it appeared independent of electric field strength. The change in proliferation rate was cell-type specific. MSCs underwent no significant change in proliferation whereas NSCs exhibited an electric field dependent response with the higher electric field producing less proliferation. Further, NSCs showed an upregulation of glial fibrillary acidic protein (GFAP) after 24 hours to 40 kV/cm, which is characteristic of astrocyte specific differentiation.
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影响因子：
3.6
作者：
Sachs, Patrick C.;Francis, Michael P.;Zhao, Min;Brumelle, Jenni;Rao, Raj R.;Elmore, Lynne W.;Holt, Shawn E.
通讯作者：
Holt, Shawn E.
星形胶质细胞和小胶质细胞的差异促炎反应涉及响应 1800 MHz 射频场的 STAT3 激活
影响因子：
3.7
作者：
Lu Y;He M;Zhang Y;Xu S;Zhang L;He Y;Chen C;Liu C;Pi H;Yu Z;Zhou Z
通讯作者：
Zhou Z
影响因子：
3.7
作者：
Kobayashi Y;Okada Y;Itakura G;Iwai H;Nishimura S;Yasuda A;Nori S;Hikishima K;Konomi T;Fujiyoshi K;Tsuji O;Toyama Y;Yamanaka S;Nakamura M;Okano H
通讯作者：
Okano H
影响因子：
2.5
作者：
Brown, JP;Couillard-Després, S;Kuhn, HG
通讯作者：
Kuhn, HG
影响因子：
3.7
作者：
Chang, Hui-Fang;Lee, Ying-Shan;Cheng, Ji-Yen
通讯作者：
Cheng, Ji-Yen