Echinococcus granulosus infection reduces airway inflammation of mice likely through enhancing IL-10 and down-regulation of IL-5 and IL-17A.
Echinococcus granulosus infection reduces airway inflammation of mice likely through enhancing IL-10 and down-regulation of IL-5 and IL-17A.
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细粒棘球绦虫感染可能通过增强 IL-10 和下调 IL-5 和 IL-17A 来减轻小鼠气道炎症
DOI：
10.1186/s13071-014-0522-6
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发表时间：
2014-11-20
影响因子：
3.2
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Wang H;Li J;Pu H;Hasan B;Ma J;Jones MK;Zheng K;Zhang X;Ma H;McManus DP;Lin R;Wen H;Zhang W
背景：囊型包虫病是由犬绦虫幼虫期细粒棘球绦虫引起的一种近乎世界性的人畜共患病。细粒棘球绦虫感染可诱导中间宿主产生极化的Th2系统免疫应答。然而，目前尚不清楚感染是否通过调节局部免疫反应来调节肺部炎症。本研究探讨细粒棘球绦虫感染对卵白蛋白(OVA)诱导的小鼠哮喘模型的影响。方法：将50个体外培养的细粒棘球绦虫小囊移植到BALB/c小鼠体内。接种后3个月，用卵白蛋白(OVA)致敏和激发小鼠。组织病理学检查采用苏木精-伊红染色和高碘酸席夫染色分别检测炎症细胞的浸润和杯状细胞的增生。用小鼠细胞珠阵列法(CBA)试剂盒和定量RT-PCR等分子生物学方法检测细胞因子水平。评估对增加剂量的乙酰甲胆碱的气道高反应性。结果：细粒棘球绦虫感染可显著提高哮喘小鼠血清和肺组织Th2、Treg细胞因子水平，下调哮喘小鼠肺组织IL-5和IL-17A的表达。肺组织组织学染色显示，细粒棘球绦虫感染可明显减轻OVA诱导的小鼠呼吸道炎症，包括嗜酸性粒细胞浸润和黏液产生减少。细粒棘球绦虫感染还可减少OVA诱导的嗜酸性粒细胞在支气管肺泡灌洗液(BALF)中的聚集，并改善哮喘的标志性症状--气道高反应性。结论：细粒棘球绦虫感染可显著减轻OVA诱导的气道炎症，其机制可能与其上调IL-10、下调IL-5和IL-17A有关。
Background:Cystic echinococcosis (CE) is a near cosmopolitan zoonosis caused by the larval stage of the dog tapeworm Echinococcus granulosus. E. granulosus infection induces a polarized T-helper type 2 (Th2) systematic immune response in its intermediate hosts. However, it is not known whether the infection modulates lung inflammation by regulating local immune response. In this study, we examined the effects of E. granulosus infection on mouse ovalbumin (OVA)-induced asthma model.Methods:BALB/c mice were intraperitoneally transplanted with 50 small E. granulosus cysts cultured in vitro. At 3 months post-inoculation, the mice were sensitized and challenged with ovalbumin (OVA). For histopathological studies, hematoxylin eosin and periodic acid schiff staining was used to examine the inflammatory cells infiltration and goblet cells hyperplasia, respectively. Cytokine levels were measured by mouse cytometric bead array (CBA) Kit and quantitative RT-PCR and other molecular biological approaches. Airway hyperresponsiveness was assessed in response to increasing doses of methacholine. Serum immunoglobulins were determined by ELISA.Results:E. granulosus infection significantly increased Th2 and Treg cytokine levels in serum and lung tissues, but down-regulated the expression of IL-5 in the lungs and IL-17A in serum and lung tissues of asthmatic mice sensitized and challenged with OVA. Histological staining of lung tissues showed that E. granulosus infection significantly reduced the severity of OVA-induced airway inflammation including reduction of eosinophil cell infiltration and mucus production. The E. granulosus infection also reduced eosinophil accumulation induced by OVA in bronchoalveolar lavage fluid (BALF) and also ameliorated airway hyperresponsiveness, a hallmark symptom of asthma.Conclusions:E. granulosus infection remarkably reduces the severity of OVA-induced airway inflammation likely through enhancing IL-10 and down-regulation of IL-5 and IL-17A.
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影响因子：
4.4
作者：
Mangan, NE;Fallon, RE;Fallon, PG
通讯作者：
Fallon, PG
DOI：
10.1016/s0167-5699(99)01551-0
发表时间：
2000-01-01
期刊：
IMMUNOLOGY TODAY
影响因子：
--
作者：
Fallon, PG
通讯作者：
Fallon, PG
影响因子：
2.2
作者：
Khabiri, A. R.;Bagheri, F.;Siavashi, M. R.
通讯作者：
Siavashi, M. R.
影响因子：
14.2
作者：
Molet, S;Hamid, Q;Chakir, J
通讯作者：
Chakir, J
影响因子：
5.4
作者：
Kim, Dae Yong;Yang, Woong Mo
通讯作者：
Yang, Woong Mo