Structure-function relationships in the adipose cell. I. Ultrastructure of the isolated adipose cell.
Structure-function relationships in the adipose cell. I. Ultrastructure of the isolated adipose cell.
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脂肪细胞中的结构 - 功能关系。 I.分离的脂肪细胞的超微结构。
DOI：
10.1083/jcb.46.2.326
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发表时间：
1970-08
影响因子：
7.8
通讯作者：
CUSHMAN, SW
中科院分区：
文献类型：
--
作者：
CUSHMAN, SW
本文介绍了一种用于电镜观察的分离大鼠脂肪细胞的制备方法。在胰岛素或肾上腺素存在下，同时研究了这种细胞的超微结构及其从U-葡萄糖-14C产生14CO_2的能力。每个脂肪细胞由一个大的脂滴组成，脂滴周围有一层薄薄的细胞质。除了典型的亚细胞器，各种小脂滴和广泛的膜系统表征细胞的细胞质。一个有孔的包膜包围着中央的大脂滴。类似的包膜包围着细胞质脂滴，这些脂滴单独存在或作为非常小的无定形液滴的聚集体存在。较小尺寸的单个液滴的组也出现在没有包膜的情况下。膜系统由细胞膜的内陷、可能是胞饮起源的小泡、简单的囊泡状空泡、细胞质深处的小泡、扁平的囊泡状光滑表面内质网和高尔基复合体组成。胰岛素和肾上腺素都没有产生可检测的超微结构改变，即使当细胞在最佳条件下孵育刺激14 CO2的演变。因此，分离的脂肪细胞对激素的结构反应，如果发生这种反应，在本质上必须是动态的，而不是定性的;表面光滑的膜的广泛系统暗示了区室化的运输和代谢。
A method is described for preparing isolated rat adipose cells for electron microscopy. The ultrastructure of such cells and their production of 14CO2 from U-glucose-14C were studied simultaneously in the presence of insulin or epinephrine. Each adipose cell consists of a large lipid droplet surrounded by a thin rim of cytoplasm. In addition to typical subcellular organelles, a variety of small lipid droplets and an extensive system of membranes characterize the cell's cytoplasm. A fenestrated envelope surrounds the large, central lipid droplet. Similar envelopes surround cytoplasmic lipid droplets occurring individually or as aggregates of very small, amorphous droplets. Groups of individual droplets of smaller size also occur without envelopes. The system of membranes consists of invaginations of the cell membrane, vesicles possibly of pinocytic origin, simple and vesiculated vacuoles, vesicles deeper in the cytoplasm, flattened and vesicular smooth surfaced endoplasmic reticulum, and Golgi complexes. Neither insulin nor epinephrine produced detectable ultrastructural alterations even when cells were incubated under optimal conditions for the stimulation of 14CO2 evolution. Structural responses of the isolated adipose cell to hormones, if such occur, must, therefore, be dynamic rather than qualitative in nature; the extensive system of smooth surfaced membranes is suggestive of compartmentalized transport and metabolism.
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脂肪细胞中的结构 - 功能关系。 ii。皮细胞增多症和影响其活性在分离的脂肪细胞中的因素。
影响因子：
7.8
作者：
Cushman, S W
通讯作者：
Cushman, S W
影响因子：
56.9
作者：
ANGEL, A
通讯作者：
ANGEL, A
影响因子：
7.7
作者：
GLIEMANN, J
通讯作者：
GLIEMANN, J
影响因子：
56.9
作者：
GOODMAN, DS
通讯作者：
GOODMAN, DS
影响因子：
3.7
作者：
MURTHY, SK
通讯作者：
MURTHY, SK