Optogenetic activation of neocortical neurons in vivo with a sapphire-based micro-scale LED probe.
Optogenetic activation of neocortical neurons in vivo with a sapphire-based micro-scale LED probe.
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DOI：
10.3389/fncir.2015.00025
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发表时间：
2015
影响因子：
3.5
通讯作者：
Mathieson K
中科院分区：
文献类型：
--
作者：
McAlinden N;Gu E;Dawson MD;Sakata S;Mathieson K
光遗传学已被证明是神经科学中的一项革命性技术，并在过去十年中不断进步。然而，需要开发用于体内的光刺激技术，以匹配推动该领域的遗传学和生物化学的进步。特别是，用于活体光学照明的传统方法在可实现的时空分辨率上具有局限性。在这里，我们利用基于蓝宝石的微型氮化镓发光二极管(μ发光二极管)探针来激活活体中的新皮质神经元。这些探头设计为包含多个独立可控的μLED，发射波长为450 nm，辐照度高达2W/mm~2。蒙特卡罗刺激预测，使用μ发光二极管的光刺激可以调节局部区域的神经活动。为了验证这一预测，我们在表达通道视紫红质-2(ChR2)的小鼠新皮质中测试了这一探针，并将结果与通过皮质表面的纤维进行的光刺激进行了比较。我们证实，这两种方法都可靠地在皮质神经元中诱导动作电位，并且μ发光二极管探针在深层神经元中引起了强烈的反应。由于可以将多个光刺激点集成到单个小腿上，因此μ发光二极管探头是一种在活体内局部控制神经元的有前途的方法。
Optogenetics has proven to be a revolutionary technology in neuroscience and has advanced continuously over the past decade. However, optical stimulation technologies for in vivo need to be developed to match the advances in genetics and biochemistry that have driven this field. In particular, conventional approaches for in vivo optical illumination have a limitation on the achievable spatio-temporal resolution. Here we utilize a sapphire-based microscale gallium nitride light-emitting diode (μLED) probe to activate neocortical neurons in vivo. The probes were designed to contain independently controllable multiple μLEDs, emitting at 450 nm wavelength with an irradiance of up to 2 W/mm2. Monte-Carlo stimulations predicted that optical stimulation using a μLED can modulate neural activity within a localized region. To validate this prediction, we tested this probe in the mouse neocortex that expressed channelrhodopsin-2 (ChR2) and compared the results with optical stimulation through a fiber at the cortical surface. We confirmed that both approaches reliably induced action potentials in cortical neurons and that the μLED probe evoked strong responses in deep neurons. Due to the possibility to integrate many optical stimulation sites onto a single shank, the μLED probe is thus a promising approach to control neurons locally in vivo.
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影响因子：
25
作者：
通讯作者：
--
影响因子：
3.6
作者：
McAlinden, Niall;Massoubre, David;Mathieson, Keith
通讯作者：
Mathieson, Keith
影响因子：
4
作者：
Wang, Jing;Wagner, Fabien;Deisseroth, Karl
通讯作者：
Deisseroth, Karl
DOI：
10.1126/science.1232437
发表时间：
2013-04-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kim TI;McCall JG;Jung YH;Huang X;Siuda ER;Li Y;Song J;Song YM;Pao HA;Kim RH;Lu C;Lee SD;Song IS;Shin G;Al-Hasani R;Kim S;Tan MP;Huang Y;Omenetto FG;Rogers JA;Bruchas MR
通讯作者：
Bruchas MR
影响因子：
2.5
作者：
Stark, Eran;Koos, Tibor;Buzsaki, Gyoergy
通讯作者：
Buzsaki, Gyoergy