Oral Administration of Bovine and Porcine Milk Exosome Alter miRNAs Profiles in Piglet Serum
Oral Administration of Bovine and Porcine Milk Exosome Alter miRNAs Profiles in Piglet Serum
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口服牛乳和猪乳外泌体改变仔猪血清中的 miRNA 谱
DOI：
10.1038/s41598-020-63485-8
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发表时间：
2020-04
影响因子：
4.6
通讯作者：
Zhang Yongliang
中科院分区：
文献类型：
--
作者：
Lin Delin;Chen Ting;Xie Meiying;Li Meng;Zeng Bin;Sun Ruiping;Zhu Yanling;Ye Dingze;Wu Jiahan;Sun Jiajie;Xi Qianyun;Jiang Qingyan;Zhang Yongliang
母乳是新生哺乳动物最重要的营养来源。研究表明，牛奶中含有microRNA（miRNAs），这是一种潜在的调节成分。目前，在奶业领域存在功能假说和营养假说，但营养假说的研究资料很少。在这项研究中，我们使用qRT-PCR方法来评估乳miRNAs是否可以通过喂养猪乳或牛乳被新生仔猪吸收。结果表明，牛乳和牛乳中miR-2284 x、2291、7134、1343、500、223的表达水平存在显著差异。4种miRNAs（miR-2284 x、2291、7134、1343）在饲喂猪乳或牛乳后的仔猪血清中差异显著。经超离心分离后，发现所筛选的miRNAs（miR-2284 x、2291、7134、1343）在乳汁中的exosomes和上清液中均有表达，且在IPEC-J2细胞中一致表达。这些发现表明，牛奶中的miRNAs在体内和体外都可以被吸收，这将为理解这类miRNAs被吸收后是否发挥生理功能奠定基础，并为营养假说提供额外的证据。
Breast milk is the most important nutrient source for newborn mammals. Studies have reported that milk contains microRNAs (miRNAs), which are potential regulatory components. Currently, existing functional and nutritional two competing hypotheses in milk field though little date have been provided for nutritional hypothesis. In this study, we used the qRT-PCR method to evaluated whether milk miRNAs can be absorbed by newborn piglets by feeding them porcine or bovine milk. The result showed that miRNA levels (miR-2284x, 2291, 7134, 1343, 500, 223) were significantly different between bovine and porcine milk. Four miRNAs (miR-2284x, 2291, 7134, 1343) were significantly different in piglet serum after feeding porcine or bovine milk. After separated milk exosomes by ultracentrifugation, the results showed the selected milk miRNAs (miR-2284x, 2291, 7134, 1343) were present in both exosomes and supernatants, and the miRNAs showed the coincidental expression in IPEC-J2 cells. All our founding suggested that the milk miRNAs can be absorbed both in vivo and in vitro, which will building the foundation for understanding whether these sort of miRNAs exert physiological functions after being absorbed and provided additional evidence for the nutritional hypotheses.
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影响因子：
4.4
作者：
Modepalli V;Kumar A;Hinds LA;Sharp JA;Nicholas KR;Lefevre C
通讯作者：
Lefevre C
DOI：
10.1186/1758-907x-1-7
发表时间：
2010-03-01
期刊：
Silence
影响因子：
--
作者：
Kosaka N;Izumi H;Sekine K;Ochiya T
通讯作者：
Ochiya T
影响因子：
7.5
作者：
Williams AH;Liu N;van Rooij E;Olson EN
通讯作者：
Olson EN
影响因子：
5
作者：
van Herwijnen MJC;Driedonks TAP;Snoek BL;Kroon AMT;Kleinjan M;Jorritsma R;Pieterse CMJ;Hoen ENMN;Wauben MHM
通讯作者：
Wauben MHM
影响因子：
3.5
作者：
Diesing, Anne-Kathrin;Nossol, Constanze;Kahlert, Stefan
通讯作者：
Kahlert, Stefan