Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell's viper venom-factor X activator (RVV-X).
Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell's viper venom-factor X activator (RVV-X).
复制标题
elapidae，viperidae和纯化的Russell的Viper毒素毒液X激活剂（RVV-X）的28种蛇毒的抗凝剂和突发性特性的比较研究。
DOI：
10.1016/j.toxicon.2010.05.012
复制
发表时间：
2010-09-15
期刊：
影响因子：
2.8
通讯作者：
Perez, John C.
中科院分区：
文献类型：
--
作者：
Suntravat, Montamas;Nuchprayoon, Issarang;Perez, John C.
关键词：
蛇毒由许多具有不同生物功能的分子组成，用于捕获猎物。毒液的每种成分都有一个特定的目标，并改变其目标的生物功能。一旦这些分子被识别、表征和克隆；它们可以用于医疗。采用活化凝血时间（ACT）和凝血率对28种蛇毒的促凝和抗凝性能进行了筛选。来自美国加州赖特伍德市的大菱鲆（Daboia russellii siamensis）、大菱鲆（Bothrops asper）、大菱鲆（Bothrops moojeni）和一种Crotalus oreganus helleri的粗毒液具有促凝活性。与阴性对照相比，这些毒液诱导ACT显著缩短，凝块率显著增加。在28种毒液中测定了因子X激活剂的活性，结果表明，siamensis的毒液比来自赖特伍德县的B. asper、B. moojeni和C. o. helleri的毒液高5-6倍。从siamensis D. r.毒液中纯化罗素毒蛇毒液因子X激活剂（RVV-X），用ACT和凝块率评价其促凝活性。其他毒液，Crotalus atrox和两种Naja pallida具有抗凝血活性。在加入这些毒液后，观察到ACT显著增加，凝块率显著降低；因此，毒液被认为具有抗凝血活性。来自同一物种的蛇毒并不总是具有相同的ACT和凝血率谱，但这些谱是鉴定蛇毒中促凝血和抗凝血活性的一种很好的方法。
Snake venoms consist of numerous molecules with diverse biological functions used for capturing prey. Each component of venom has a specific target, and alters the biological function of its target. Once these molecules are identified, characterized, and cloned; they could have medical applications. The activated clotting time (ACT) and clot rate were used for screening procoagulant and anticoagulant properties of 28 snake venoms. Crude venoms from Daboia russellii siamensis, Bothrops asper, Bothrops moojeni, and one Crotalus oreganus helleri from Wrightwood, CA, had procoagulant activity. These venoms induced a significant shortening of the ACT and showed a significant increase in the clot rate when compared to the negative control. Factor X activator activity was also measured in 28 venoms, and D. r. siamensis venom was 5–6 times higher than those of B. asper, B. moojeni, and C. o. helleri from Wrightwood County. Russell's viper venom-factor X activator (RVV-X) was purified from D. r. siamensis venom, and then procoagulant activity was evaluated by the ACT and clot rate. Other venoms, Crotalus atrox and two Naja pallida, had anticoagulant activity. A significant increase in the ACT and a significant decrease in the clot rate were observed after the addition of these venoms; therefore, the venoms were considered to have anticoagulant activity. Venoms from the same species did not always have the same ACT and clot rate profiles, but the profiles were an excellent way to identify procoagulant and anticoagulant activities in snake venoms.
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影响因子：
2.9
作者：
HOFMANN, H;BON, C
通讯作者：
BON, C
DOI：
10.1016/j.cbpc.2006.03.001
发表时间：
2006-07-01
影响因子：
3.9
作者：
Gowda, C. D. Raghavendra;Nataraju, A.;Vishwanath, B. S.
通讯作者：
Vishwanath, B. S.
影响因子：
2.8
作者：
BAJWA, SS;MARKLAND, FS;RUSSELL, FE
通讯作者：
RUSSELL, FE
影响因子：
6.7
作者：
ARONSON, DL
通讯作者：
ARONSON, DL
影响因子：
2.8
作者：
FARID, T;NASSER, H;ELASMAR, MF
通讯作者：
ELASMAR, MF