Corneal confocal microscopy identifies corneal nerve fibre loss and increased dendritic cells in patients with long COVID.
Corneal confocal microscopy identifies corneal nerve fibre loss and increased dendritic cells in patients with long COVID.
复制标题
DOI：
10.1136/bjophthalmol-2021-319450
复制
发表时间：
2022-12
期刊：
影响因子：
--
通讯作者：
Malik RA
中科院分区：
文献类型：
--
作者：
Bitirgen G;Korkmaz C;Zamani A;Ozkagnici A;Zengin N;Ponirakis G;Malik RA
长期COVID的特点是一系列潜在的衰弱症状，至少有10%的人从急性SARS-CoV-2感染中康复。这项研究量化了患有和未患有长期COVID的患者的角膜基底下神经丛形态和树突状细胞（DC）密度。这项在大学医院进行的横断面比较研究包括40名从COVID-19中康复的受试者和30名对照参与者。所有患者都接受了美国国家健康与护理卓越研究所（NICE）长期COVID、Douleur Neuropathique 4（DN 4）和纤维肌痛问卷以及角膜共焦显微镜（CCM）的评估，以量化角膜神经纤维密度（CNFD）、角膜神经分支密度（CNBD）、角膜神经纤维长度（CNFL）以及总的、成熟的和未成熟的DC密度。确诊COVID-19后的平均时间为3.7±1.5个月。与对照组相比，急性COVID-19后4周有神经系统症状的患者CNFD（p=0.032）、CNBD（p=0.020）和CNFL（p=0.012）较低，DC密度增加（p=0.046），而无神经系统症状的患者角膜神经参数相似，但DC密度增加（p=0.003）。在第4周和第12周，NICE长COVID问卷的总分与CNFD（分别为ρ=-0.436; p=0.005，ρ=-0.387; p=0.038）和CNFL（分别为ρ=-0.404; p=0.010，ρ=-0.412; p=0.026）之间存在显著相关性。角膜共聚焦显微镜可识别长期COVID患者，特别是有神经症状的患者的角膜小神经纤维丢失和DC增加。CCM可用于客观识别长期COVID患者。
Long COVID is characterised by a range of potentially debilitating symptoms which develop in at least 10% of people who have recovered from acute SARS-CoV-2 infection. This study has quantified corneal sub-basal nerve plexus morphology and dendritic cell (DC) density in patients with and without long COVID. Forty subjects who had recovered from COVID-19 and 30 control participants were included in this cross-sectional comparative study undertaken at a university hospital. All patients underwent assessment with the National Institute for Health and Care Excellence (NICE) long COVID, Douleur Neuropathique 4 (DN4) and Fibromyalgia questionnaires, and corneal confocal microscopy (CCM) to quantify corneal nerve fibre density (CNFD), corneal nerve branch density (CNBD), corneal nerve fibre length (CNFL), and total, mature and immature DC density. The mean time after the diagnosis of COVID-19 was 3.7±1.5 months. Patients with neurological symptoms 4 weeks after acute COVID-19 had a lower CNFD (p=0.032), CNBD (p=0.020), and CNFL (p=0.012), and increased DC density (p=0.046) compared with controls, while patients without neurological symptoms had comparable corneal nerve parameters, but increased DC density (p=0.003). There were significant correlations between the total score on the NICE long COVID questionnaire at 4 and 12 weeks with CNFD (ρ=−0.436; p=0.005, ρ=−0.387; p=0.038, respectively) and CNFL (ρ=−0.404; p=0.010, ρ=−0.412; p=0.026, respectively). Corneal confocal microscopy identifies corneal small nerve fibre loss and increased DCs in patients with long COVID, especially those with neurological symptoms. CCM could be used to objectively identify patients with long COVID.
登录
查看更多内容
COVID-19 的 50 多种长期影响：系统回顾和荟萃分析。
影响因子：
4.6
作者：
Lopez-Leon S;Wegman-Ostrosky T;Perelman C;Sepulveda R;Rebolledo PA;Cuapio A;Villapol S
通讯作者：
Villapol S
影响因子：
5.3
作者：
Stettner, Mark;Hinrichs, Lena;Kieseier, Bernd C.
通讯作者：
Kieseier, Bernd C.
影响因子：
5.3
作者：
Tavakoli, Mitra;Marshall, Andrew;Malik, Rayaz A.
通讯作者：
Malik, Rayaz A.
影响因子：
3.7
作者：
Kalteniece, Alise;Ferdousi, Maryam;Malik, Rayaz A.
通讯作者：
Malik, Rayaz A.
影响因子：
7.4
作者：
Shiers S;Ray PR;Wangzhou A;Sankaranarayanan I;Tatsui CE;Rhines LD;Li Y;Uhelski ML;Dougherty PM;Price TJ
通讯作者：
Price TJ