Unilateral inactivation of the basolateral amygdala attenuates context-induced renewal of Pavlovian-conditioned alcohol-seeking.
Unilateral inactivation of the basolateral amygdala attenuates context-induced renewal of Pavlovian-conditioned alcohol-seeking.
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DOI：
10.1111/ejn.12278
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Janak PH
中科院分区：
文献类型：
--
作者：
Chaudhri N;Woods CA;Sahuque LL;Gill TM;Janak PH
与药物使用相关的环境环境促进人类的渴望和动物的药物寻求。我们假设基底外侧杏仁核（BLA）本身以及基底外侧杏仁核（BLA）和伏隔核（NAC核）之间的串联连接是情境诱导的巴普洛夫条件下的酒精寻求更新所必需的。雄性Long-Evans大鼠被训练来区分两种条件刺激（CS）——一种是与乙醇（乙醇，20%,v/v）递送到液体端口（0.2 ml/CS+， 3.2 ml/次）配对的CS+，另一种是没有条件刺激的CS -。测量每次CS期间进入端口的入口。接下来，在没有EtOH的情况下，大鼠在不同的环境中接受了灭绝。在测试中，在先前的训练环境中评估CS+和CS -没有EtOH的反应。对照受试者的CS+相对于灭绝表现出选择性的增加，表明再生。该效应通过预测阻断，使用γ -氨基丁酸受体激动剂溶液（0.1 mM muscimol和1.0 mM巴氯芬；M/B； 0.3 μ l/侧）使BLA双侧失活。单侧向BLA注射M/B，联合M/B、多巴胺D1受体拮抗剂SCH 23390（0.6µg/侧）或对侧NAC核心输注生理盐水后，更新也减弱。因此，单侧BLA失活足以破坏更新，突出了BLA功能活动在酒精环境下恢复酒精寻求的关键作用。
Environmental contexts associated with drug use promote craving in humans and drug-seeking in animals. We hypothesized that the basolateral amygdala (BLA) itself, as well as serial connectivity between the basolateral amygdala (BLA) and nucleus accumbens core (NAC core), were required for context-induced renewal of Pavlovian-conditioned alcohol-seeking. Male, Long-Evans rats were trained to discriminate between two conditioned stimuli (CS) - a CS+ that was paired with ethanol (EtOH, 20%, v/v) delivery into a fluid port (0.2 ml/CS+, 3.2 ml/session) and a CS− that was not. Entries into the port during each CS were measured. Next, rats received extinction in a different context where both cues were presented without EtOH. At test, responding to the CS+ and CS− without EtOH was evaluated in the prior training context. Control subjects showed a selective increase in CS+ responding relative to extinction, indicative of renewal. This effect was blocked by pre-test, bilateral inactivation of the BLA using a solution of gamma-amino-butyric-acid receptor agonists (0.1 mM muscimol and 1.0 mM baclofen; M/B; 0.3 µl/side). Renewal was also attenuated following unilateral injections of M/B into the BLA, combined with either M/B, the dopamine D1 receptor antagonist SCH 23390 (0.6 µg/side), or saline infusion in the contralateral NAC core. Hence, unilateral BLA inactivation was sufficient to disrupt renewal, highlighting a critical role for functional activity in the BLA in enabling the reinstatement of alcohol-seeking driven by an alcohol context.
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DOI：
10.1111/j.1460-9568.2008.06517.x
发表时间：
2008-12
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Chaudhri N;Sahuque LL;Cone JJ;Janak PH
通讯作者：
Janak PH
DOI：
10.1111/j.1460-9568.2009.07044.x
发表时间：
2010-01
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Gremel CM;Cunningham CL
通讯作者：
Cunningham CL
DOI：
10.1038/npp.2009.187
发表时间：
2010-02
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.3
作者：
EVERITT, BJ;MORRIS, KA;ROBBINS, TW
通讯作者：
ROBBINS, TW
影响因子：
5.3
作者：
Floresco, SB;Ghods-Sharifi, S;Magyar, O
通讯作者：
Magyar, O