Identification of a 130-kilodalton human biliary concanavalin A binding protein as aminopeptidase N.
Identification of a 130-kilodalton human biliary concanavalin A binding protein as aminopeptidase N.
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将 130 千道尔顿的人胆汁伴刀豆球蛋白 A 结合蛋白鉴定为氨肽酶 N。
DOI：
10.1016/0016-5085(94)90712-9
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发表时间：
1994
期刊：
影响因子：
29.4
通讯作者：
Afdhal,NH
中科院分区：
文献类型：
--
作者：
Offner,GD;Gong,D;Afdhal,NH
背景/目的：人的胆汁含有一组与凝集素刀豆蛋白A(ConA)结合的非粘蛋白糖蛋白，已有报道促进胆固醇一水结晶成核，这是胆结石形成之前的事件。其中几种蛋白质，包括一种130千道尔顿的蛋白质，已经被分离出来，并被证明在体外可以促进核形成。方法：胆汁经ConA琼脂糖层析后，电泳层析、印迹、氨基末端序列分析。结果：主要ConA结合蛋白分别为氨基肽酶N(130kodalton蛋白)、α2巨球蛋白、血凝蛋白、免疫球蛋白重链和结合珠蛋白β链。进一步纯化后，发现氨基肽酶N在胆汁中具有酶活性，并能促进胆固醇结晶，其生理浓度与胆汁中胆固醇的生理浓度相近。结论：氨基肽酶N很可能是先前鉴定的130kodalton胆汁结晶促进蛋白。氨基肽酶N可能通过胆盐的去污剂活性从胆小管膜中释放出来，可能是促进胆汁中胆固醇结晶的因素之一。
Background/Aims:Human gallbladder bile contains a group of nonmucin glycoproteins that binds to the lectin concanavalin A (con A) and has been reported to promote cholesterol monohydrate crystal nucleation, an event preceding the formation of gallstones. Several of these proteins, including a 130-kilodalton protein, have been isolated and shown to promote nucleation in vitro. The aim of this study was to identify this and other major biliary con A binding glycoproteins.Methods:Gallbladder bile was chromatographed on con A agarose, and the eluted proteins were electrophoresed, blotted, and subjected to amino-terminal sequence analysis.Results:The major con A binding proteins were identified as aminopeptidase N (a 130-kilodalton protein), α2 macroglobulin, hemopexin, immunoglobulin heavy chains, and the β chain of haptoglobin. After further purification, aminopeptidase N was found to be enzymatically active and to promote cholesterol crystallization at its approximate physiological concentration in bile.Conclusions:It is likely that aminopeptidase N is the previously characterized 130-kilodalton biliary crystallization promoting protein. Aminopeptidase N is probably released from the biliary canalicular membrane by the detergent activity of bile salts and may be one factor that promotes cholesterol crystallization in the gallbladder.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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