Identification of the main venom protein components of Aphidius ervi, a parasitoid wasp of the aphid model Acyrthosiphon pisum.
Identification of the main venom protein components of Aphidius ervi, a parasitoid wasp of the aphid model Acyrthosiphon pisum.
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DOI：
10.1186/1471-2164-15-342
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发表时间：
2014-05-06
期刊：
影响因子：
4.4
通讯作者：
Gatti JL
中科院分区：
文献类型：
--
作者：
Colinet D;Anselme C;Deleury E;Mancini D;Poulain J;Azéma-Dossat C;Belghazi M;Tares S;Pennacchio F;Poirié M;Gatti JL
内寄生蜂是广泛分布的蚜虫害虫的重要天敌，主要用作生物防治剂。然而，尽管蚜虫相互作用网络的兴趣越来越大，只有稀疏的信息是可用的寄生蜂用来调节蚜虫生理的因素。我们的目的是在这里确定的主要蛋白质成分注射在产卵的蚜ervi，以确保成功的发展，在其蚜虫宿主，Acyrthosiphon豌豆。结合大规模的转录组学和蛋白质组学的方法，使我们能够确定16个推定的毒液蛋白，其中三个γ-谷氨酰转肽酶（γ-GT）是迄今为止最丰富的。其中两个γ-GT最有可能对应于同一基因的等位基因，其中一个等位基因先前被描述为参与宿主去势。第三个γ-GT仅与其他γ-GT有远亲关系，并且由于活性位点中存在突变而可能不具有功能。在毒液中其他丰富的蛋白质中，有几种是A. ervi，如分子伴侣胞质内溶酶，可能参与保护蛋白质在宿主中的分泌和运输。丰富的转录本编码三个分泌的富含半胱氨酸的毒素样肽，其功能仍有待探讨。我们的数据进一步支持γ-GT作为A. ervi对蚜虫寄主的影响。然而，他们也证明这种黄蜂毒液是一种复杂的液体，含有不同的，或多或少的特定蛋白质成分。它们的特征无疑将有助于解释寄生虫-蚜虫和寄生虫-蚜虫-共生体的相互作用。最后，这项研究还揭示了毒液成分的快速进化过程，如复制和收敛招聘的毒力因子之间不相关的生物。本文的在线版本（doi：10.1186/1471-2164-15-342）包含补充材料，可供授权用户使用。
Endoparasitoid wasps are important natural enemies of the widely distributed aphid pests and are mainly used as biological control agents. However, despite the increased interest on aphid interaction networks, only sparse information is available on the factors used by parasitoids to modulate the aphid physiology. Our aim was here to identify the major protein components of the venom injected at oviposition by Aphidius ervi to ensure successful development in its aphid host, Acyrthosiphon pisum. A combined large-scale transcriptomic and proteomic approach allowed us to identify 16 putative venom proteins among which three γ-glutamyl transpeptidases (γ-GTs) were by far the most abundant. Two of the γ-GTs most likely correspond to alleles of the same gene, with one of these alleles previously described as involved in host castration. The third γ-GT was only distantly related to the others and may not be functional owing to the presence of mutations in the active site. Among the other abundant proteins in the venom, several were unique to A. ervi such as the molecular chaperone endoplasmin possibly involved in protecting proteins during their secretion and transport in the host. Abundant transcripts encoding three secreted cystein-rich toxin-like peptides whose function remains to be explored were also identified. Our data further support the role of γ-GTs as key players in A. ervi success on aphid hosts. However, they also evidence that this wasp venom is a complex fluid that contains diverse, more or less specific, protein components. Their characterization will undoubtedly help deciphering parasitoid-aphid and parasitoid-aphid-symbiont interactions. Finally, this study also shed light on the quick evolution of venom components through processes such as duplication and convergent recruitment of virulence factors between unrelated organisms. The online version of this article (doi:10.1186/1471-2164-15-342) contains supplementary material, which is available to authorized users.
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影响因子：
12.3
作者：
Gerardo NM;Altincicek B;Anselme C;Atamian H;Barribeau SM;de Vos M;Duncan EJ;Evans JD;Gabaldón T;Ghanim M;Heddi A;Kaloshian I;Latorre A;Moya A;Nakabachi A;Parker BJ;Pérez-Brocal V;Pignatelli M;Rahbé Y;Ramsey JS;Spragg CJ;Tamames J;Tamarit D;Tamborindeguy C;Vincent-Monegat C;Vilcinskas A
通讯作者：
Vilcinskas A
DOI：
10.1093/bioinformatics/btp024
发表时间：
2009-03-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Gouzy J;Carrere S;Schiex T
通讯作者：
Schiex T
影响因子：
2.2
作者：
Caccia, Silvia;Grimaldi, Annalisa;Giordana, Barbara
通讯作者：
Giordana, Barbara
影响因子：
3.8
作者：
Falabella, Patrizia;Riviello, Lea;Pennacchio, Francesco
通讯作者：
Pennacchio, Francesco
影响因子：
3.8
作者：
Falabella, Patrizia;Riviello, Lea;Pennacchio, Francesco
通讯作者：
Pennacchio, Francesco