Extraction optimization by response surface methodology, purification and principal antioxidant metabolites of red pigments extracted from bayberry (Myrica rubra) pomace
Extraction optimization by response surface methodology, purification and principal antioxidant metabolites of red pigments extracted from bayberry (Myrica rubra) pomace
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杨梅果渣红色素的响应面法提取优化、纯化及主要抗氧化代谢物
DOI：
10.1016/j.lwt.2012.09.029
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发表时间：
2013-04
影响因子：
6
通讯作者：
Xiao W. X.
中科院分区：
文献类型：
--
作者：
Zhang Y. L.;Kong L. C.;Yin C. P.;Jiang D. H.;Jiang J. Q.;He J.;Xiao W. X.
采用响应面法优化杨梅果渣中红色素的提取工艺条件为：pH值1.4，提取时间4.2 h，提取温度56.1 °C，液料比12.6/1 ml/g。采用大孔吸附树脂对RP进行纯化，结果表明LX-68树脂的纯化效果最好。RP进一步溶剂分配得到乙酸乙酯、正丁醇和水层，其中第一级分清除DPPH和羟自由基的活性最强，IC 50值分别为2.13 μg/ml和0.57 mg/ml，高于阳性对照的BHA和甘露醇。经活性导向分离，从乙酸乙酯层中分离得到（-）-表儿茶素和原花青素B2，它们具有良好的DPPH自由基清除活性，IC 50值分别为2.87和2.06 μg/ml。
The optimized extraction conditions of red pigments (RP) from bayberry (Myrica rubra) pomace obtained with response surface methodology were extraction pH, time, temperature, liquid/solid ratio as 1.4, 4.2 h, 56.1 °C and 12.6/1 ml/g, respectively. The RP were then purified by the macroporous resin, which indicated the resin LX-68 showed the maximum refined efficiency among the tested resins. Further solvent partition of the RP yielded ethyl acetate, n-butanol and water layers, in which the first fraction showed the strongest DPPH and hydroxyl radical scavenging activities with IC50values of 2.13 μg/ml and 0.57 mg/ml, respectively, which were higher than those of BHA and mannitol used as a positive control. Following activity-oriented separation, (−)-epicatechin and procyanidin B2 were isolated from the ethyl acetate layer and were found to have excellent DPPH free radical scavenging activities with IC50values of 2.87 and 2.06 μg/ml, respectively.
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