A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications.
A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications.
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DOI：
10.1016/j.bios.2018.04.002
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发表时间：
2018-10-15
影响因子：
12.6
通讯作者：
Blain Christen JM
中科院分区：
文献类型：
--
作者：
Obahiagbon U;Smith JT;Zhu M;Katchman BA;Arafa H;Anderson KS;Blain Christen JM
对抗传染病的一种有效方法是在护理点（POC）部署手持式设备，用于筛查或自我监测应用。需要非常灵敏、低成本和定量的诊断装置。在这项研究中，我们提出了一种低成本，多路复用的荧光检测平台，具有高灵敏度和宽动态范围。我们的系统具有廉价的3×3 mm干涉滤波器，具有高阻带抑制，陡峭的过渡边缘，通带内的透射率大于90%。除了滤波器之外，我们还通过利用基于电荷积分的读出来利用时间提高精度，从而提高信噪比。该荧光传感平台对光子通量的灵敏度为101 ×104光子/mm 2秒，与使用彩色乳胶微球的标准比色检测相比，灵敏度有可能提高2至3个数量级。我们还详细介绍了我们的低成本荧光检测平台的设计，开发和表征，并证明串扰概率和滤波器成本分别降低了100%和97.96%。这是通过在2×2阵列配置中减小前端尺寸并确保适当的通道隔离来实现的。低成本干扰FI系统的设计，分析和系统性能的实际考虑也进行了讨论。我们的平台的性能相比，一个标准的实验室阵列扫描仪。我们还证明了人乳头瘤病毒（HPV 16）E7蛋白抗体的检测，作为一个潜在的生物标志物，在人血浆中的早期宫颈癌检测。
An effective method of combating infectious diseases is the deployment of hand-held devices at the point-of-care (POC) for screening or self-monitoring applications. There is a need for very sensitive, low-cost and quantitative diagnostic devices. In this study, we present a low-cost, multiplexed fluorescence detection platform that has a high sensitivity and wide dynamic range. Our system features inexpensive 3×3 mm interference filter with a high stopband rejection, sharp transition edges, and greater than 90% transmission in the passband. In addition to the filters we improve signal-to-noise ratio by leveraging time for accuracy using a charge-integration-based readout. The fluorescence sensing platform provides a sensitivity to photon flux of ∼1×104 photons/mm2sec and has the potential for 2 to 3 orders of magnitude improvement in sensitivity over standard colorimetric detection that uses colored latex microspheres. We also detail the design, development, and characterization of our low-cost fluorescence detection platform and demonstrate 100% and 97.96% reduction in crosstalk probability and filter cost, respectively. This is achieved by reducing fi r dimensions and ensuring appropriate channel isolation in a 2×2 array configuration. Practical considerations with low-cost interference fi system design, analysis, and system performance are also discussed. The performance of our platform is compared to that of a standard laboratory array scanner. We also demonstrate the detection of antibodies to human papillomavirus (HPV16) E7 protein, as a potential biomarker for early cervical cancer detection in human plasma.
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影响因子：
8.4
作者：
Lefevre, Florent;Juneau, Philippe;Izquierdo, Ricardo
通讯作者：
Izquierdo, Ricardo
影响因子：
6.1
作者：
Ryu, Gihan;Huang, Jingsong;Bradley, Donal D. C.
通讯作者：
Bradley, Donal D. C.
影响因子：
4.6
作者：
Katchman BA;Smith JT;Obahiagbon U;Kesiraju S;Lee YK;O'Brien B;Kaftanoglu K;Blain Christen J;Anderson KS
通讯作者：
Anderson KS
影响因子：
--
作者：
Smith, Joseph T.;Katchman, Benjamin A.;Christen, Jennifer Blain
通讯作者：
Christen, Jennifer Blain
用于荧光横向流量测定的低成本高性能系统。
DOI：
10.3390/bios3040360
发表时间：
2013-10-21
期刊：
Biosensors
影响因子：
--
作者：
Lee LG;Nordman ES;Johnson MD;Oldham MF
通讯作者：
Oldham MF