Realignment of signal processing within a sensory brainstem nucleus as brain temperature declines in the Syrian hamster, a hibernating species.
Realignment of signal processing within a sensory brainstem nucleus as brain temperature declines in the Syrian hamster, a hibernating species.
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DOI：
10.1007/s00359-011-0706-x
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发表时间：
2012-04
影响因子：
2.1
通讯作者：
Chen, Chao-Yin
中科院分区：
文献类型：
--
作者：
Sekizawa, Shin-Ichi;Horowitz, John M.;Horwitz, Barbara A.;Chen, Chao-Yin
对于生存至关重要的是，中枢神经系统必须在冬眠的所有阶段可靠地处理感觉信息，以确保维持体内平衡调节，并与急剧变化的行为状态相匹配。通过比较大鼠和恒温叙利亚仓鼠孤束核的神经反应，我们验证了仓鼠孤束核神经元在保存能量的同时具有维持信号处理的适应性的假设。利用膜片钳技术，我们将二级孤束核神经元根据其对去极化脉冲（在−80 mV预脉冲之后）的峰值发作分为快速发作或延迟发作的峰值表型。当温度从33℃降至15℃时，所有神经元的兴奋性均下降。在各温度下，仓鼠快起神经元的峰值反应最高，动作电位宽度最短，而仓鼠延迟起神经元的静息膜电位负性最大。两种表型的自发性兴奋性突触后电流频率都随温度的降低而降低，但无论表型和温度如何，仓鼠的孤束刺激诱发的兴奋性突触后电流振幅都大于大鼠。变化显著（P<0.05），支持我们的假设，表明随着温度下降，快速发作神经元对信号处理的贡献比延迟发作神经元大，但对能量保存的贡献比延迟发作神经元小。
Crucial for survival, the central nervous system must reliably process sensory information over all stages of a hibernation bout to ensure homeostatic regulation is maintained and well-matched to dramatically altered behavioral states. Comparing neural responses in the nucleus tractus solitarius of rats and euthermic Syrian hamsters, we tested the hypothesis that hamster nucleus tractus solitarius neurons have adaptations sustaining signal processing while conserving energy. Using patch-clamp techniques, we classified second-order nucleus tractus solitarius neurons as rapid-onset or delayed-onset spiking phenotypes based on their spiking-onset to a depolarizing pulse (following a −80 mV prepulse). As temperature decreased from 33°C to 15°C, the excitability of all neurons decreased. However, hamster rapid-onset neurons had the highest spiking response and shortest action potential width at every temperature, while hamster delayed-onset neurons had the most negative resting membrane potential. Spontaneous excitatory post-synaptic current frequency in both phenotypes decreased as temperature decreased, yet tractus solitarius stimulation-evoked excitatory post-synaptic current amplitudes were greater in hamsters than in rats regardless of phenotype and temperature. Changes were significant (P<0.05), supporting our hypothesis by showing that, as temperature falls, rapid-onset neurons contribute more to signal processing but less to energy conservation than do delayed-onset neurons.
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影响因子：
--
作者：
HORWITZ, BA;SMITH, RE;PENGELLEY, ET
通讯作者：
PENGELLEY, ET
影响因子：
3.3
作者：
BELOUSOV, AB;VINOGRADOVA, OS;PAKHOTIN, PI
通讯作者：
PAKHOTIN, PI
影响因子：
3.3
作者：
Gabriel, A;Klussmann, FW;Igelmund, P
通讯作者：
Igelmund, P
影响因子：
3.3
作者：
Kline, D. D.;King, T. L.;Austgen, J. R.;Heesch, C. M.;Hasser, E. M.
通讯作者：
Hasser, E. M.
影响因子：
5.5
作者：
HODGKIN, AL;KATZ, B
通讯作者：
KATZ, B