Characterization of axons expressing the artemin receptor in the female rat urinary bladder: a comparison with other major neuronal populations.
Characterization of axons expressing the artemin receptor in the female rat urinary bladder: a comparison with other major neuronal populations.
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DOI：
10.1002/cne.23648
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发表时间：
2014-12-01
影响因子：
2.5
通讯作者：
Keast, Janet R.
中科院分区：
文献类型：
--
作者：
Forrest, Shelley L.;Osborne, Peregrine B.;Keast, Janet R.
青蒿素是神经胶质细胞系来源的神经营养因子（GDNF）家族的一员，在自主神经的发育和再生以及伤害感觉的调节中有重要作用。虽然该家族的其他成员（GDNF和neurturin）主要作用于副交感神经和非肽能感觉神经元，但artemin受体（GFRα3）由交感神经和肽能感觉神经元表达，这些神经元也是神经生长因子（膀胱神经的强大调节剂）的主要作用位点。许多膀胱感觉神经元表达GFRα3，但不知道它们是否代表一个特定的功能亚类。因此，我们最初的目的是绘制gfr α3免疫反应（-IR）轴突在雌性大鼠膀胱中的分布，使用低温切片和全壁厚制备。我们发现GFRα3-IR轴突支配逼尿肌、脉管系统和尿路上皮，但这种支配仅部分是感觉神经支配。支配血管的去肾上腺素能交感神经轴突多为GFRα3-IR，而支配逼尿肌的去肾上腺素能神经轴突为gfr α3阴性。我们还确定了非神经元GFRα3-IR的一个重要来源，可能是胶质细胞。膀胱神经的进一步表征揭示了化学上不同种类的轴突末端的特定结构特征，以及神经丝-200标记的轴突的主要自主来源，这通常用于识别器官内有髓鞘的感觉轴突。内膜神经元也被表征和量化。总之，这些研究揭示了青蒿素可能影响膀胱功能、神经再生和疼痛的多种潜在靶点，并为理解膀胱生理学和病理生理学提供了强有力的微观解剖学框架。
Artemin is a member of the glial cell line-derived neurotrophic factor (GDNF) family that has been strongly implicated in development and regeneration of autonomic nerves, and modulation of nociception. Whereas other members of this family (GDNF and neurturin) primarily target parasympathetic and non-peptidergic sensory neurons, the artemin receptor (GFRα3) is expressed by sympathetic and peptidergic sensory neurons that are also the primary sites of action of nerve growth factor, a powerful modulator of bladder nerves. Many bladder sensory neurons express GFRα3 but it is not known if they represent a specific functional subclass. Therefore, our initial aim was to map the distribution of GFRα3-immunoreactive (-IR) axons in the female rat bladder, using cryostat sections and whole wall thickness preparations. We found that GFRα3-IR axons innervated the detrusor, vasculature and urothelium, but only part of this innervation was sensory. Many noradrenergic sympathetic axons innervating the vasculature were GFRα3-IR, but the noradrenergic innervation of the detrusor was GFRα3-negative. We also identified a prominent source of non-neuronal GFRα3-IR that is likely to be glial. Further characterisation of bladder nerves revealed specific structural features of chemically distinct classes of axon terminals, and a major autonomic source of axons labelled with neurofilament-200, which is commonly used to identify myelinated sensory axons within organs. Intramural neurons were also characterised and quantified. Together, these studies reveal a diverse range of potential targets by which artemin could influence bladder function, nerve regeneration and pain, and provide a strong micro-anatomical framework for understanding bladder physiology and pathophysiology.
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DOI：
10.1016/s0165-1838(97)00032-5
发表时间：
1997-07-14
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
CallsenCencic, P;Mense, S
通讯作者：
Mense, S
影响因子：
2.5
作者：
Chao, Tom;Pham, Khoa;Gupta, Ranjan
通讯作者：
Gupta, Ranjan
影响因子：
3.8
作者：
Bennett, David L. H.;Boucher, Timothy J.;McMahon, Stephen B.
通讯作者：
McMahon, Stephen B.
影响因子：
--
作者：
ELBADAWI, A;SCHENK, EA
通讯作者：
SCHENK, EA
影响因子：
6.6
作者：
Davidson, RA;McCloskey, KD
通讯作者：
McCloskey, KD