Development of a MR-visible compound for tracing neuroanatomical connections in vivo.
Development of a MR-visible compound for tracing neuroanatomical connections in vivo.
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DOI：
10.1016/j.neuron.2011.03.010
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发表时间：
2011-04-28
期刊：
影响因子：
16.2
通讯作者：
Ungerleider LG
中科院分区：
文献类型：
--
作者：
Wu CW;Vasalatiy O;Liu N;Wu H;Cheal S;Chen DY;Koretsky AP;Griffiths GL;Tootell RB;Ungerleider LG
Traditional studies of neuroanatomical connections require injection of tracer compounds into living brains, then histology of the post-mortem tissue. Here we describe and validate a new compound that reveals neuronal connections in vivo, using MRI. The classic anatomical tracer CTB (cholera-toxin subunit-B) was conjugated with a gadolinium-chelate to form GdDOTA-CTB. GdDOTA-CTB was injected into the primary somatosensory cortex (S1) or the olfactory pathway of rats. High-resolution MR images were collected at a range of time points at 11.7T and 7T. The transported GdDOTA-CTB was visible for at least 1 month post-injection, clearing within two months. Control injections of non-conjugated GdDOTA into S1 were not transported, and cleared within 1–2 days. Control injections of Gd-Albumin were not transported either, clearing within 7 days. These MR results were verified by classic immunohistochemical staining for CTB, in the same animals. The GdDOTA-CTB neuronal transport was target-specific, monosynaptic, stable for several weeks, and reproducible.
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影响因子：
3.4
作者：
Brubaker CJ;Schmithorst VJ;Haynes EN;Dietrich KN;Egelhoff JC;Lindquist DM;Lanphear BP;Cecil KM
通讯作者：
Cecil KM
早期视觉皮层中额叶信号的自下而上的门控。
DOI：
10.1126/science.1153276
发表时间：
2008-07-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ekstrom LB;Roelfsema PR;Arsenault JT;Bonmassar G;Vanduffel W
通讯作者：
Vanduffel W
影响因子：
3
作者：
Astary, Garrett W.;Kantorovich, Svetlana;Carney, Paul R.;Mareci, Thomas H.;Sarntinoranont, Malisa
通讯作者：
Sarntinoranont, Malisa
影响因子：
3
作者：
ERICSON, H;BLOMQVIST, A
通讯作者：
BLOMQVIST, A
影响因子：
2.5
作者：
Akers, RM;Killackey, HP
通讯作者：
Killackey, HP