Interstitial Cajal-like cells (ICLC) in atrial myocardium: ultrastructural and immunohistochemical characterization.
Interstitial Cajal-like cells (ICLC) in atrial myocardium: ultrastructural and immunohistochemical characterization.
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DOI：
10.1111/j.1582-4934.2006.tb00306.x
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发表时间：
2006-01
影响因子：
5.3
通讯作者：
Popescu LM
中科院分区：
文献类型：
--
作者：
Hinescu ME;Gherghiceanu M;Mandache E;Ciontea SM;Popescu LM
我们以前曾报道过（Hinescu和Popescu，2005），通过透射电子显微镜观察，在人类心房肌中存在间质Cajal样细胞（ICLC）。在本研究中，ICLC通过非常规光学显微镜（NCLM）在甲苯胺蓝染色的半薄切片上进行鉴定，并通过免疫组化（IHC）检测CD 117/c-kit、CD 34、波形蛋白和其他用于鉴别诊断的额外抗原。在半薄切片上，定量地，ICLC约占心房心肌体积的1-1.5%（相对于±45%的工作肌细胞，约2%的内皮细胞，3-4%的其他间质细胞，其余百分比：细胞外基质）。大致上，在心外膜下方的细胞间隙中存在一个ICLC，用于8-10个工作心房肌细胞，具有特征性（梨形、梭形或三角形）形状。这些ICLC通常有2-3个明确的突起，从细胞体中出现，通常包围心房肌细胞（质膜/肌膜平均距离260 nm）或与神经纤维或毛细血管建立紧密接触（与内皮细胞平均距离约420 nm）。细胞形态特征为：极薄（平均厚度为0.150±0.1 μm），非神经细胞极长（数十μm），念珠状（直径不均匀）。在ICLC和其他间质细胞（巨噬细胞）之间发现了基质突触（例如，在多接触型突触中，平均突触间隙为1.65 nm）。自然地，通常的细胞器（线粒体、平滑和粗糙内质网、中间丝）相对发达。细胞膜上也可见小窝。未检测到粗丝。免疫组化结果显示，ICLC中CD 117/c-kit呈弱阳性，CD 34和EGF受体呈不同程度的共表达，波形蛋白呈强阳性，波形蛋白沿着排列。部分ICLC表达α-平滑肌肌动蛋白和tau蛋白，但不表达nestin、desmin、CD 13和S-100。总之，我们提供了ICLC在人类心房心肌中存在的进一步证据，支持ICLC在起搏、分泌（分泌和/或旁分泌）、细胞间信号传导（神经元和肌细胞）中可能的作用。从病理学上看，ICLC可能是心房重构的参与者。
We have previously reported (Hinescu & Popescu, 2005) the existence of interstitial Cajal-like cells (ICLC), by transmission electron microscopy, in human atrial myocardium. In the present study, ICLC were identified with non-conventional light microscopy (NCLM) on semi-thin sections stained with toluidine blue and immunohistochemistry (IHC) for CD117/c-kit, CD34, vimentin and other additional antigens for differential diagnosis. Quantitatively, on semi-thin sections, ICLC represent about 1–1.5% of the atrial myocardial volume (vs.±45% working myocytes, ˜2% endothelial cells, 3–4% for other interstitial cells, and the remaining percentage: extracellular matrix). Roughly, there is one ICLC for 8–10 working atrial myocytes in the intercellular space, beneath the epicardium, with a characteristic (pyriform, spindle or triangular) shape. These ICLC usually have 2–3 definitory processes, emerging from cell body, which usually embrace atrial myocytes (260 nm average distance plasmalemma/sarcolemma) or establish close contact with nerve fibers or capillaries (˜420 nm average distance to endothelial cells). Cell prolongations are characteristic: very thin (mean thickness = 0.150±0.1 μm), very long for a non-nervous cell (several tens of μm) and moniliform (uneven caliber). Stromal synapses between ICLC and other interstitial cells (macrophages) were found (e.g. in a multicontact type synapse, the average synaptic cleft was ˜65 nm). Naturally, the usual cell organelles (mitochondria, smooth and rough endoplasmic reticulum, intermediate filaments) are relatively well developed. Caveolae were also visible on cell prolongations. No thick filaments were detected. IHC showed that ICLC were slightly and inconsistently positive for CD117/c-kit, variously co-expressed CD34 and EGF receptor, but appeared strongly positive for vimentin, along their prolongations. Some ICLC seemed positive for α-smooth muscle actin and tau protein, but were negative for nestin, desmin, CD13 and S-100. In conclusion, we provide further evidence of the existence of ICLC in human atrial myocardium, supporting the possible ICLC role in pacemaking, secretion (juxta- and/or paracrine), intercellular signaling (neurons and myocytes). For pathology, ICLC might as well be ‘players’ in arrhythmogenesis and atrial remodeling.
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影响因子：
3.6
作者：
Duquette, RA;Shmygol, A;Wray, S
通讯作者：
Wray, S
影响因子：
3.6
作者：
Bobryshev, YV
通讯作者：
Bobryshev, YV
影响因子：
6.6
作者：
Davidson, RA;McCloskey, KD
通讯作者：
McCloskey, KD
影响因子：
5.5
作者：
Bradley, E;Hollywood, MA;Sergeant, GP
通讯作者：
Sergeant, GP
DOI：
10.1111/j.1582-4934.2005.tb00387.x
发表时间：
2005-10-01
影响因子：
5.3
作者：
Gherghiceanu, M;Popescu, LM
通讯作者：
Popescu, LM