Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor.
Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor.
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DOI：
10.1016/j.bios.2021.113206
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发表时间：
2021-07-01
影响因子：
12.6
通讯作者：
Zhang GJ
中科院分区：
文献类型：
--
作者：
Li J;Wu D;Yu Y;Li T;Li K;Xiao MM;Li Y;Zhang ZY;Zhang GJ
SARS-CoV-2RNA被认为是支持新冠肺炎检测的能源区的关键因素。在此，我们开发了一种快速、非扩增的纳米传感平台，用于检测人类咽拭子标本中的SARS-CoV-2RNA。制备了金纳米颗粒修饰的石墨烯场效应管(G-FET)传感器，并在其表面固定了互补的磷二酰吗啉寡核苷酸(PMO)探针。该传感器可用于SARS-CoV-2 RdRp的高灵敏度测试，因为PMO不带电荷，导致背景信号低。该方法不仅对PBS(0.37Fm)、咽拭子(2.29Fm)和血清(3.99Fm)的检测下限较低，而且在2分钟内对新冠肺炎患者的样本实现了快速反应。所研制的纳米传感器能够分析30个真实临床样本的RNA提取液。结果表明，该传感器能够区分健康人和感染者，与RT-PCR结果高度一致(Kappa指数=40.92)。此外，还对SARS-CoV-2 RdRp和SARS-CoV RdRp进行了明确的区分。因此，我们认为这项工作为新冠肺炎的诊断提供了一个令人满意的、有吸引力的选择。
SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G-FET) sensor was fabricated, after which complementary phosphorodiamidate morpholino oligos (PMO) probe was immobilized on the AuNP surface. This sensor allowed for highly sensitive testing of SARS-CoV-2 RdRp as PMO does not have charges, leading to low background signal. Not only did the method present a low limit of detection in PBS (0.37 fM), throat swab (2.29 fM), and serum (3.99 fM), but also it achieved a rapid response to COVID-19 patients’ samples within 2 min. The developed nanosensor was capable of analyzing RNA extracts from 30 real clinical samples. The results show that the sensor could differentiate the healthy people from infected people, which are in high agreement with RT-PCR results (Kappa index = 0.92). Furthermore, a well-defined distinction between SARS-CoV-2 RdRp and SARS-CoV RdRp was also made. Therefore, we believe that this work provides a satisfactory, attractive option for COVID-19 diagnosis.
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