Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex
Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex
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精确定位小鼠视觉皮层兴奋性神经元的形态和投射
DOI：
10.3389/fnins.2019.00912
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发表时间：
2019-08
影响因子：
4.3
通讯作者：
Chen Shangbin
中科院分区：
文献类型：
--
作者：
Zhang Yalun;Jiang Siqi;Xu Zhengchao;Gong Hui;Li Man;Luo Qingming;Ren Miao;Li Xiangning;Wu Hao;Yuan Jing;Chen Shangbin
视觉皮层的兴奋性神经元对于我们理解大脑功能具有重要意义。然而，不同的神经元类型及其形态特性尚未完全破译。在本文中，我们应用全脑定位系统（BPS）以 0.2 μm × 0.2 μm × 1 μm 体素分辨率对两只 Thy1-eYFP H 系雄性小鼠的整个大脑进行成像。在视觉皮层第 5 层和第 6 层总共重建了 103 个神经元，具有单轴突级分辨率。基于神经元的完整拓扑结构和成像方法固有的定位功能，我们根据其顶端树突和体细胞位置将观察到的神经元分为六种类型：第5层星状锥体细胞（L5-sp）、第5层细长簇状锥体细胞（L5-st）、第5层簇状锥体细胞（L5-tt）、第6层刺状星状细胞（L6-ss）、第 6 层有星形锥体细胞 (L6-sp)，第 6 层有细长簇状锥体细胞 (L6-st)。通过检查单个神经元的轴突投射模式，可以将它们分为三种模式：同侧回路连接神经元、胼胝体投射神经元和离皮质投射神经元。关联这两种类型的分类，我们发现除了L5-tt之外，之前定义的神经元类型中至少包含两种投影模式。另一方面，每种投影模式可能由本研究中定义的四种树突类型组成。轴突投影模式仅与顶端树突特征部分相关。这项工作展示了通过单神经元水平量化解析视觉皮层的范例，并显示出扩展以揭示其他定义的感觉和运动系统的连接组的潜力。
The excitatory neurons in the visual cortex are of great significance for us in understanding brain functions. However, the diverse neuron types and their morphological properties have not been fully deciphered. In this paper, we applied the brain-wide positioning system (BPS) to image the entire brain of two Thy1-eYFP H-line male mice at 0.2 μm × 0.2 μm × 1 μm voxel resolution. A total of 103 neurons were reconstructed in layers 5 and 6 of the visual cortex with single-axon-level resolution. Based on the complete topology of neurons and the inherent positioning function of the imaging method, we classified the observed neurons into six types according to their apical dendrites and somata location: star pyramidal cells in layer 5 (L5-sp), slender-tufted pyramidal cells in layer 5 (L5-st), tufted pyramidal cells in layer 5 (L5-tt), spiny stellate-like cells in layer 6 (L6-ss), star pyramidal cells in layer 6 (L6-sp), and slender-tufted pyramidal cells in layer 6 (L6-st). By examining the axonal projection patterns of individual neurons, they can be categorized into three modes: ipsilateral circuit connection neurons, callosal projection neurons and corticofugal projection neurons. Correlating the two types of classifications, we have found that there are at least two projection modes comprised in the former defined neuron types except for L5-tt. On the other hand, each projection mode may consist of four dendritic types defined in this study. The axon projection mode only partially correlates with the apical dendrite feature. This work has demonstrated a paradigm for resolving the visual cortex through single-neuron-level quantification and has shown potential to be extended to reveal the connectome of other defined sensory and motor systems.
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影响因子：
25
作者：
Zhang, Siyu;Xu, Min;Chang, Wei-Cheng;Ma, Chenyan;Johnny Phong Hoang Do;Jeong, Daniel;Lei, Tiffany;Fan, Jiang Lan;Dan, Yang
通讯作者：
Dan, Yang
影响因子：
16.2
作者：
Kim EJ;Juavinett AL;Kyubwa EM;Jacobs MW;Callaway EM
通讯作者：
Callaway EM
视觉皮层单细胞投射的逻辑。
影响因子：
64.8
作者：
Han Y;Kebschull JM;Campbell RAA;Cowan D;Imhof F;Zador AM;Mrsic-Flogel TD
通讯作者：
Mrsic-Flogel TD
影响因子：
7.7
作者：
Albanese A;Chung K
通讯作者：
Chung K
影响因子：
25
作者：
Roth MM;Dahmen JC;Muir DR;Imhof F;Martini FJ;Hofer SB
通讯作者：
Hofer SB