Higher diversity of ammonia/ammonium-oxidizing prokaryotes in constructed freshwater wetland than natural coastal marine wetland.
Higher diversity of ammonia/ammonium-oxidizing prokaryotes in constructed freshwater wetland than natural coastal marine wetland.
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DOI：
10.1007/s00253-012-4430-4
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发表时间：
2013-08
影响因子：
5
通讯作者：
Gu, Ji-Dong
中科院分区：
文献类型：
--
作者：
Wang, Yong-Feng;Gu, Ji-Dong
关键词：
厌氧氨氧化 (anammox) 细菌、需氧氨氧化古菌 (AOA) 和氨氧化细菌 (AOB) 是参与氮循环的三类氨/氨氧化原核生物 (AOP)。这项研究比较了香港亚热带人工淡水湿地和天然沿海海洋湿地中的 AOP 群落。对植被/根际和非植被沉积物进行了研究，以确定不同大型植物对 AOP 群落的影响。应用聚合酶链式反应 (PCR) 扩增的 16S rRNA 以及古菌和细菌 amoA（编码氨单加氧酶 α 亚基）的基因片段作为分子生物标志物来分析 AOP 的系统发育和多样性。定量 PCR 用于测定沉积物中 AOP 的丰度。结果表明，异质性相对较高的淡水湿地比滨海湿地具有更广泛的系统发育型、更高的多样性、更复杂的群落结构以及更不均匀的AOPs丰度分布。植被对 AOP 群落结构的影响是植物特异性的。外来的香蒲影响了所有 AOP 的群落结构，并提高了它们在根际区域的丰度。芦苇和莎草对 AOB 的群落结构有一定影响，但对厌氧氨氧化菌或 AOA 的群落结构影响很小。由于秋茄尺寸较小，因此对所有 AOP 几乎没有可检测到的影响。本研究表明，由于AOP群落和植物类型的差异，淡水和沿海海洋湿地对无机氮去除的贡献可能不同。本文的在线版本 (doi:10.1007/s00253-012-4430-4) 包含补充材料，可供授权用户使用。
Anaerobic ammonium-oxidizing (anammox) bacteria, aerobic ammonia-oxidizing archaea (AOA), and ammonia-oxidizing bacteria (AOB) are three groups of ammonium/ammonia-oxidizing prokaryotes (AOPs) that are involved in the nitrogen cycle. This research compared the AOP communities in a constructed freshwater wetland with a natural coastal marine wetland in the subtropical Hong Kong. Both vegetated/rhizosphere and nonvegetated sediments were investigated to identify the effects of different macrophytes on the AOP communities. The polymerase chain reaction (PCR)-amplified gene fragments of 16S rRNA and archaeal and bacterial amoA (encoding the ammonia monooxygenase alpha subunit) were applied as molecular biomarkers to analyze the AOPs’ phylogeny and diversity. Quantitative PCR was used to determine the abundances of AOPs in the sediments. The results showed that the relatively more heterogeneous freshwater wetland contained a broader range of phylotypes, higher diversity, more complex community structures, and more unevenly distributed abundances of AOPs than the coastal wetland. The effects of vegetation on the community structures of AOPs were plant-specific. The exotic Typha angustifolia affected the community structures of all AOPs and enhanced their abundances in the rhizosphere region. Both Phragmites australis and Cyperus malaccensis showed some effects on the community structures of AOB, but minimal effects on those of anammox bacteria or AOA. Kandelia obovata had almost no detectable effect on all AOPs due to their smaller size. This study suggested that the freshwater and coastal marine wetlands may have different contributions to the inorganic N removal due to the variations in AOP communities and plant types. The online version of this article (doi:10.1007/s00253-012-4430-4) contains supplementary material, which is available to authorized users.
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