Enzyme-mediated redox initiation for hydrogel generation and cellular encapsulation.
Enzyme-mediated redox initiation for hydrogel generation and cellular encapsulation.
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DOI：
10.1021/bm900846m
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发表时间：
2009-11-09
影响因子：
6.2
通讯作者：
Bowman, Christopher N.
中科院分区：
文献类型：
--
作者：
Johnson, Leah M.;Fairbanks, Benjamin D.;Anseth, Kristi S.;Bowman, Christopher N.
一种涉及葡萄糖氧化酶和Fe+2的快速、水溶性酶介导的自由基链引发体系在25°C和常氧条件下几分钟内就产生了水凝胶。用近红外光谱研究了引发组分对丙烯酸羟乙酯-聚乙二醇575-二丙烯酸酯共聚单体溶液聚合速率的影响。随着葡萄糖浓度的增加，聚合速率加快，直至达到1×10−3M以上的速率平台。在Fe+21.0×10−4M和5.0×10−4M范围内，初始聚合速率与Fe+2浓度呈平方根关系，过量的Fe+2降低了丙烯酸酯的最终转化率。利用葡萄糖氧化酶介导的引发体系将成纤维细胞(NIH3T3s)包裹到聚乙二醇四丙烯酸酯(Mn20,000)水凝胶支架中，包裹后24小时的存活率为96%(±3%)。首次将酶介导的氧化还原自由基链引发用于细胞包埋，展示了水凝胶的原位聚合和动力学控制，最小的氧抑制问题和低引发剂浓度的利用。
A rapid, water-soluble enzyme-mediated radical chain initiation system involving glucose oxidase and Fe+2 generated hydrogels within minutes at 25°C and in ambient oxygen. The initiation components were evaluated for their effect on polymerization rates of hydroxyethyl acrylate-poly(ethylene glycol)575 diacrylate comonomer solutions using near-infrared spectroscopy. Increasing glucose concentration increased polymerization rates until reaching a rate plateau above 1 × 10−3M of glucose. A square root dependence of the initial polymerization rate on Fe+2 concentration was observed between 1.0 × 10−4M and 5.0 × 10−4M of Fe+2 whereupon excess Fe+2 reduced final acrylate conversions. The glucose oxidase-mediated initiation system was employed for encapsulation of fibroblasts (NIH3T3s) into a poly(ethylene glycol) tetra-acrylate (Mn~20,000) hydrogel scaffold demonstrating 96% (±3%) viability at 24 hours post-encapsulation. This first use of enzyme-mediated redox radical chain initiation for cellular encapsulation demonstrates polymerization of hydrogels in situ with kinetic control, minimal oxygen inhibition issues and utilization of low initiator concentrations.
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影响因子：
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