ITRAQ-based proteomic analysis of apple buds provides new insights into regulatory mechanisms of flowering in response to shoot bending
ITRAQ-based proteomic analysis of apple buds provides new insights into regulatory mechanisms of flowering in response to shoot bending
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基于 ITRAQ 的苹果芽蛋白质组学分析为响应枝条弯曲的开花调节机制提供了新的见解
DOI：
10.1016/j.scienta.2021.110526
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发表时间：
2021-12
影响因子：
4.3
通讯作者：
Xing Libo
中科院分区：
文献类型：
--
作者：
Liu Sitong;Shi Ting;Zhang Danni;Zhang Xiaochen;Fan Tianle;Mu Pengcheng;Yue Haiying;Li Xiaolong;Tian Jianwen;Xing Libo
开花是苹果树生命周期的一个重要阶段，幼龄的“永富2号”只会产生有限的次花蕾。茎弯是一种有效的促进开花的农艺措施，但其分子机制尚不清楚。采用等压标签进行相对定量和绝对定量的LC - MS/MS，鉴定了花芽分化过程中花芽中表达的8,585个蛋白。将弯曲后的1060个上调蛋白和1079个下调蛋白分为7个模式组：I（22/5）、II（30/57）、III（34/21）、IV（77/61）、V（381/452）、VI（218/170）和VII（298/313）。蛋白质的数量与碳水化合物、脂肪酸和脂质代谢以及激素、转录因子、昼夜节律、光周期和开花途径有关。茎部弯曲导致脂肪酸和脂质途径的差异表达蛋白上调，而其他淀粉合成相关蛋白下调。此外，茎弯还上调了与昼夜节律和开花相关的基因。本研究提供了一份有价值的花芽发育相关蛋白清单，有助于理解苹果芽弯曲引起花芽分化的调控机制。
Flowering is an important life cycle stage in apple trees, and young ‘Nagafu No. 2’ produce limited inferior flower buds. Shoot bending, an effective agronomic measure, is widely used to promote flowering but little is known regarding the underlying molecular mechanisms. Using isobaric tags for relative and absolute quantification LC−MS/MS, 8,585 proteins expressed in buds during flower bud differentiation were identified. The 1,060 up- and 1,079 down-regulated proteins after shoot bending were divided into 7 pattern-based groups: I (22/5), II (30/57), III (34/21), IV (77/61), V (381/452), VI (218/170), and VII (298/313) types. Number of proteins were associated with carbohydrate, fatty acid, and lipid metabolism, as well as hormone, transcription factor, circadian rhythm, and photoperiod and flowering pathways. The differentially expressed proteins involved in fatty acid and lipid pathways were up-regulated by shoot bending, while other proteins that were involved in starch synthesis were down-regulated. In addition, circadian rhythm- and flowering-related genes were up-regulated by shoot bending. This research provides a valuable inventory of proteins involved in floral bud development and contributes to the understanding of the regulatory mechanisms of flower bud differentiation caused by shoot bending in apple trees.
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影响因子：
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作者：
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影响因子：
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作者：
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