Isolating neural correlates of the pacemaker for food anticipation.
Isolating neural correlates of the pacemaker for food anticipation.
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DOI：
10.1371/journal.pone.0036117
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Abizaid A
中科院分区：
文献类型：
--
作者：
Blum ID;Lamont EW;Rodrigues T;Abizaid A
在昼夜节律范围内每隔一段时间喂食一顿饭的小鼠表现出食物预期活动。以前的研究有力地表明，这种行为受到禁食/重新进食时间的昼夜节律起搏器的调节。这种起搏器的神经关联(S)，食物携带振荡器(FEO)，无论是在神经网络中还是在单个基因座上都是未知的。这项研究使用了昼夜节律起搏器的典型特性，即在去除携带刺激后继续振荡的能力，将食物携带振荡器神经关联中的激活与驱动食物预期活动的所有其他机制分开。有人假设，在限制进食和恢复自由进食后，中央核持续的预期激活将阐明负责食物携带振荡器计时组件的信号电路的神经表示。动物被带到临时受限的食物中，然后被放回自由进食几天，直到食物预期活动被取消。通过对c-fos表达细胞的体视学分析，对整个大脑的核团的激活进行了量化，并与随意喂养和食物携带的对照组进行了比较。几个下丘脑和脑干核团在前一次食物预期时仍处于激活状态，这表明它们参与了跟踪先前食物呈现所需的计时机制。这项研究还为进一步研究FEO的解剖学和分子基础的实验范式提供了概念证明。
Mice fed a single daily meal at intervals within the circadian range exhibit food anticipatory activity. Previous investigations strongly suggest that this behaviour is regulated by a circadian pacemaker entrained to the timing of fasting/refeeding. The neural correlate(s) of this pacemaker, the food entrainable oscillator (FEO), whether found in a neural network or a single locus, remain unknown. This study used a canonical property of circadian pacemakers, the ability to continue oscillating after removal of the entraining stimulus, to isolate activation within the neural correlates of food entrainable oscillator from all other mechanisms driving food anticipatory activity. It was hypothesized that continued anticipatory activation of central nuclei, after restricted feeding and a return to ad libitum feeding, would elucidate a neural representation of the signaling circuits responsible for the timekeeping component of the food entrainable oscillator. Animals were entrained to a temporally constrained meal then placed back on ad libitum feeding for several days until food anticipatory activity was abolished. Activation of nuclei throughout the brain was quantified using stereological analysis of c-FOS expressing cells and compared against both ad libitum fed and food entrained controls. Several hypothalamic and brainstem nuclei remained activated at the previous time of food anticipation, implicating them in the timekeeping mechanism necessary to track previous meal presentation. This study also provides a proof of concept for an experimental paradigm useful to further investigate the anatomical and molecular substrates of the FEO.
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DOI：
10.1152/ajpregu.1998.274.5.r1309
发表时间：
1998-05-01
影响因子：
2.8
作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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作者：
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