The role of shear dynamics in biofilm formation.
The role of shear dynamics in biofilm formation.
复制标题
剪切动力学在生物膜形成中的作用。
DOI：
10.1038/s41522-022-00300-4
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发表时间：
2022-04-29
影响因子：
9.2
通讯作者：
Sloan, William T.
中科院分区：
文献类型：
--
作者：
Tsagkari, Erifyli;Connelly, Stephanie;Liu, Zhaowei;McBride, Andrew;Sloan, William T.
越来越多的证据表明，单个细菌能感知并对机械负荷的变化做出反应。然而，多物种生物膜对动态流体剪切应力的微妙反应并没有得到很好的记录，因为实验经常不能将剪切应力的任何有益影响与重要营养物质的对流运输所带来的有益影响分开。我们观察了饮用水中具有对数正态分布的微菌落大小的生物膜的发展，这些生物膜位于日益复杂的流动状态下的流道壁上。首先，有规则的涡旋诱导了振荡壁剪切，同时增强了质量传递，从而产生了最厚最广泛的生物膜。第二，非定常均匀流动施加振荡壁面剪切，没有增强的输运，生物量和盖度仅减少20%。最后，对于具有恒定壁面剪切的均匀稳定流动，生物膜的范围、厚度和密度平均小60%。因此，剪切应力的动态作用在促进生物膜发育中起着重要的作用，超过其大小或传质效应，因此，机械传感可能在复杂的多物种生物膜中普遍存在，这可能为控制生物膜结构开辟新的途径。
There is growing evidence that individual bacteria sense and respond to changes in mechanical loading. However, the subtle responses of multispecies biofilms to dynamic fluid shear stress are not well documented because experiments often fail to disentangle any beneficial effects of shear stress from those delivered by convective transport of vital nutrients. We observed the development of biofilms with lognormally distributed microcolony sizes in drinking water on the walls of flow channels underflow regimes of increasing complexity. First, where regular vortices induced oscillating wall shear and simultaneously enhanced mass transport, which produced the thickest most extensive biofilms. Second, where unsteady uniform flow imposed an oscillating wall shear, with no enhanced transport, and where the biomass and coverage were only 20% smaller. Finally, for uniform steady flows with constant wall shear where the extent, thickness, and density of the biofilms were on average 60% smaller. Thus, the dynamics of shear stress played a significant role in promoting biofilm development, over and above its magnitude or mass transfer effects, and therefore, mechanosensing may prevail in complex multispecies biofilms which could open up new ways of controlling biofilm structure.
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影响因子：
5.2
作者：
Fish KE;Boxall JB
通讯作者：
Boxall JB
影响因子：
6.3
作者：
Miranda AF;Ramkumar N;Andriotis C;Höltkemeier T;Yasmin A;Rochfort S;Wlodkowic D;Morrison P;Roddick F;Spangenberg G;Lal B;Subudhi S;Mouradov A
通讯作者：
Mouradov A
影响因子：
3.9
作者：
Rusconi, Roberto;Lecuyer, Sigolene;Stone, Howard A.
通讯作者：
Stone, Howard A.
影响因子：
3.8
作者：
Garny, Kerstin;Horn, Harald;Neu, Thomas R.
通讯作者：
Neu, Thomas R.
影响因子：
4.4
作者：
Battin, TJ;Kaplan, LA;Hansen, C
通讯作者：
Hansen, C