The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for bluetongue virus.
The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for bluetongue virus.
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DOI：
10.1186/s12864-018-5014-1
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发表时间：
2018-08-22
期刊：
影响因子：
4.4
通讯作者：
Fife M
中科院分区：
文献类型：
--
作者：
Morales-Hojas R;Hinsley M;Armean IM;Silk R;Harrup LE;Gonzalez-Uriarte A;Veronesi E;Campbell L;Nayduch D;Saski C;Tabachnick WJ;Kersey P;Carpenter S;Fife M
新的基因组技术为载体、病毒和宿主之间相互作用的遗传学提供了新的见解，这导致了具有医学重要性的虫媒病毒控制方面的进展。然而，非人畜共患虫媒病毒的载体的工具和资源的开发仍然被忽视。库蠓属的叮咬蠓传播一些世界范围内野生动物和牲畜的最重要的虫媒病毒，对经济生产力、健康和福利产生全球性影响。合适的参考基因组的缺乏阻碍了迄今为止在这一重要的载体属中的基因组分析。在本研究中，在美国的蓝舌病病毒（BTV）的载体库蠓sonorensis的基因组，已被测序，为这些载体提供第一个参考基因组。在这项研究中，我们还报告了使用参考基因组进行初始转录组学分析的载体能力BTV。我们的分析表明，基因组为189 Mb，组装在7974个支架中。使用本研究和先前研究中生成的转录组数据进行注释，已确定了15，612个基因。C.在本研究中进行的感染BTV的sonorensis雌性揭示了165个基因在载体感受态和难治性雌性之间差异表达。两个候选基因，谷胱甘肽S-转移酶（gst）和抗病毒解旋酶ski 2，以前被认为是参与载体的能力，BTV在C。sonorensis（gst）和抑制dsRNA病毒繁殖（ski 2）。C. sonorensis已经能够初步分析载体感受态和难治个体的基因表达谱。本研究所获得的基因组和转录组为今后虫媒病毒传播的研究提供了合适的工具。这些为这些媒介谱系提供了宝贵的资源，这些谱系在2亿多年前从其他主要的双翅目媒介家族中分化出来。该基因组将是其他重要的双翅目媒介家庭，包括蚊子（蚊科）和白蛉（Psychodidae）的比较数据的宝贵来源，并与转录组数据一起可以产生潜在的目标，转基因改造的载体控制和功能研究。本文的在线版本（10.1186/s12864-018-5014-1）包含补充材料，可供授权用户使用。
The new genomic technologies have provided novel insights into the genetics of interactions between vectors, viruses and hosts, which are leading to advances in the control of arboviruses of medical importance. However, the development of tools and resources available for vectors of non-zoonotic arboviruses remains neglected. Biting midges of the genus Culicoides transmit some of the most important arboviruses of wildlife and livestock worldwide, with a global impact on economic productivity, health and welfare. The absence of a suitable reference genome has hindered genomic analyses to date in this important genus of vectors. In the present study, the genome of Culicoides sonorensis, a vector of bluetongue virus (BTV) in the USA, has been sequenced to provide the first reference genome for these vectors. In this study, we also report the use of the reference genome to perform initial transcriptomic analyses of vector competence for BTV. Our analyses reveal that the genome is 189 Mb, assembled in 7974 scaffolds. Its annotation using the transcriptomic data generated in this study and in a previous study has identified 15,612 genes. Gene expression analyses of C. sonorensis females infected with BTV performed in this study revealed 165 genes that were differentially expressed between vector competent and refractory females. Two candidate genes, glutathione S-transferase (gst) and the antiviral helicase ski2, previously recognized as involved in vector competence for BTV in C. sonorensis (gst) and repressing dsRNA virus propagation (ski2), were confirmed in this study. The reference genome of C. sonorensis has enabled preliminary analyses of the gene expression profiles of vector competent and refractory individuals. The genome and transcriptomes generated in this study provide suitable tools for future research on arbovirus transmission. These provide a valuable resource for these vector lineage, which diverged from other major Dipteran vector families over 200 million years ago. The genome will be a valuable source of comparative data for other important Dipteran vector families including mosquitoes (Culicidae) and sandflies (Psychodidae), and together with the transcriptomic data can yield potential targets for transgenic modification in vector control and functional studies. The online version of this article (10.1186/s12864-018-5014-1) contains supplementary material, which is available to authorized users.
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影响因子：
2.1
作者：
JONES, RH;FOSTER, NM
通讯作者：
FOSTER, NM
影响因子：
5.8
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DOI：
10.20506/rst.34.1.2347
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通讯作者：
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