A microporous surface containing Si(3)N(4)/Ta microparticles of PEKK exhibits both antibacterial and osteogenic activity for inducing cellular response and improving osseointegration.
A microporous surface containing Si(3)N(4)/Ta microparticles of PEKK exhibits both antibacterial and osteogenic activity for inducing cellular response and improving osseointegration.
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含有 PEKK Si3N4/Ta 微粒的微孔表面具有抗菌和成骨活性，可诱导细胞反应并改善骨整合
DOI：
10.1016/j.bioactmat.2021.02.027
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发表时间：
2021-10
影响因子：
18.9
通讯作者：
Wei J
中科院分区：
文献类型：
--
作者：
Hu X;Mei S;Wang F;Tang S;Xie D;Ding C;Du W;Zhao J;Yang L;Wu Z;Wei J
作为一种可植入的生物材料，聚醚酮酮（PEKK）具有良好的机械强度，但它是生物惰性的，而钽（Ta）具有突出的成骨生物活性，但具有高密度和弹性模量。此外，氮化硅（SN）具有成骨和抗菌活性。在这项研究中，通过磺化作用在PEKK（STP）上形成了一个含有SN和Ta微粒的微孔表面，该表面表现出优异的成骨和抗菌活性。与不含微粒的磺化PEKK（SPK）相比，由于微孔表面存在SN和Ta颗粒，STP的表面性质（粗糙度、表面能、亲水性和蛋白质吸附）显着增加。此外，STP还表现出优异的抗菌活性，由于SN颗粒的存在，其在体外抑制细菌生长并在体内防止细菌感染。此外，含有SN和Ta颗粒的STP的微孔表面显著地诱导响应（例如，增殖和分化）的大鼠骨髓间充质干细胞（rBMS）。此外，STP显著改善体内新骨再生和骨整合。在体外诱导细胞反应和体内改善骨整合方面，含Ta的微孔表面优于含SN颗粒的表面。总之，具有优化表面特性的STP在体外激活细胞反应，增强骨整合并预防体内感染。因此，STP具有良好的成骨和抗菌双重生物学功能，显示出作为骨替代材料的巨大潜力。·在PEKK（STP）上产生含有SN/Ta微粒的微孔表面。·表面性能（例如，粗糙度）的STP显著增加。·STP在体外表现出抗菌活性，并在体内防止感染。·STP在体外可显著诱导骨髓间充质干细胞的反应。·STP明显促进体内骨再生和骨整合。
As an implantable biomaterial, polyetherketoneketone (PEKK) exhibits good mechanical strength but it is biologically inert while tantalum (Ta) possesses outstanding osteogenic bioactivity but has a high density and elastic modulus. Also, silicon nitride (SN) has osteogenic and antibacterial activity. In this study, a microporous surface containing both SN and Ta microparticles on PEKK (STP) exhibiting excellent osteogenic and antibacterial activity was created by sulfonation. Compared with sulfonated PEKK (SPK) without microparticles, the surface properties (roughness, surface energy, hydrophilicity and protein adsorption) of STP significantly increased due to the SN and Ta particles presence on the microporous surface. In addition, STP also exhibited outstanding antibacterial activity, which inhibited bacterial growth in vitro and prevented bacterial infection in vivo because of the presence of SN particles. Moreover, the microporous surface of STP containing both SN and Ta particles remarkably induced response (e.g., proliferation and differentiation) of rat bone mesenchymal stem (rBMS) cells in vitro. Furthermore, STP significantly improved new bone regeneration and osseointegration in vivo. Regarding the induction of cellular response in vitro and improvement of osseointegration in vivo, the microporous surface containing Ta was better than the surface with SN particles. In conclusion, STP with optimized surface properties activated cellular responses in vitro, enhanced osseointegration and prevented infection in vivo. Therefore, STP possessed the dual biofunctions of excellent osteogenic and antibacterial activity, showing great potential as a bone substitute. •Microporous surface containing SN/Ta microparticles on PEKK (STP) was created. •Surface performances (e.g., roughness) of STP were significantly increased. •STP exhibited antibacterial activity in vitro and prevented infection in vivo. •STP remarkably induced response of bone mesenchymal stem cells in vitro. •STP obviously improved bone regeneration and osseointegration in vivo.
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