Optimization of Xylanase production from Penicillium sp.WX-Z1 by a two-step statistical strategy: Plackett-Burman and Box-Behnken experimental design.
Optimization of Xylanase production from Penicillium sp.WX-Z1 by a two-step statistical strategy: Plackett-Burman and Box-Behnken experimental design.
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DOI：
10.3390/ijms130810630
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发表时间：
2012
影响因子：
5.6
通讯作者：
Zhao L
中科院分区：
文献类型：
--
作者：
Cui F;Zhao L
采用Plackett-Burman设计和Box-Behnken设计对青霉WX-Z1产木聚糖酶培养基中的营养源进行优化。首先采用Plackett-Burman多因子设计筛选青霉WX-Z1产木聚糖酶的重要营养源，然后采用Box-Behnken设计进一步优化响应面法（RSM）。在培养基中的重要营养源，确定由Placket-Burman的初步筛选方法，是麦麸，酵母提取物，NaNO 3，MgSO 4，CaCl 2。最佳添加量（g/L）为：麦麸32.8、酵母膏1.02、NaNO 3 12.71、MgSO 4 0.96、CaCl 2 1.04。在30 L的生物反应器中进行发酵试验，在优化条件下，木聚糖酶的产量达到46.50U/mL，比原培养基提高了1.34倍。
The objective of the study was to optimize the nutrition sources in a culture medium for the production of xylanase from Penicillium sp.WX-Z1 using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the important nutrient sources in the medium for xylanase production by Penicillium sp.WX-Z1 and subsequent use of the response surface methodology (RSM) was further optimized for xylanase production by Box-Behnken design. The important nutrient sources in the culture medium, identified by the initial screening method of Placket-Burman, were wheat bran, yeast extract, NaNO3, MgSO4, and CaCl2. The optimal amounts (in g/L) for maximum production of xylanase were: wheat bran, 32.8; yeast extract, 1.02; NaNO3, 12.71; MgSO4, 0.96; and CaCl2, 1.04. Using this statistical experimental design, the xylanase production under optimal condition reached 46.50 U/mL and an increase in xylanase activity of 1.34-fold was obtained compared with the original medium for fermentation carried out in a 30-L bioreactor.
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