Human and Xenopus cDNAs encoding budding yeast Cdc7‐related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7
Human and Xenopus cDNAs encoding budding yeast Cdc7‐related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7
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编码芽殖酵母 Cdc7 相关激酶的人类和非洲爪蟾 cDNA：假定的 Cdc7 人类同源物对 MCM 亚基的体外磷酸化
DOI：
10.1093/emboj/16.14.4340
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
H. Masai
中科院分区：
文献类型：
--
作者：
N. Sato;K. Arai;H. Masai
酿酒酵母Cdc7激酶是启动DNA复制所必需的，裂殖酵母的相关激酶Hsk1也是裂殖酵母细胞DNA复制所必需的。我们在此报告了编码人类和非洲爪蟾Cdc7相关激酶的cDNA（分别为huCdc7和xeCdc7）。克隆的huCdc7 cDNA包含一个由574个氨基酸组成的开放阅读框架，预测分子量为63 847，与Cdc7和Hsk 1的氨基酸同源性为32%（54%包括相似残基）。huCDC 7在所检查的各种组织中转录，但在睾丸中最丰富。检测到长度为4.4、3.5和2.4 kb的三种转录物。3.5 kb的转录本是最主要的，并在所有检查的组织中表达。还检测到在huCDC 7的N末端区域含有91个核苷酸插入的cDNA，表明存在多个剪接变体。huCdc7蛋白在有丝分裂细胞周期期间以恒定水平表达，并且主要定位于间期的细胞核中，并且在有丝分裂期扩散性地分布于细胞质中。在COS7细胞中表达的野生型huCdc7蛋白在体外磷酸化MCM2和MCM3蛋白，表明huCdc7可能通过调节MCM功能来调节DNA复制过程。
Saccharomyces cerevisiae Cdc7 kinase is essential for initiation of DNA replication, and Hsk1, a related kinase of Schizosaccharomyces pombe, is also required for DNA replication of fission yeast cells. We report here cDNAs encoding Cdc7‐related kinases from human and Xenopus (huCdc7 and xeCdc7, respectively). The cloned cDNA for huCdc7 contains an open reading frame consisting of 574 amino acids with a predicted molecular weight of 63 847 that possesses overall amino acid identity of 32% (54% including similar residues) to Cdc7 and Hsk1. huCDC7 is transcribed in the various tissues examined, but most abundantly in testis. Three transcripts of 4.4, 3.5 and 2.4 kb in length are detected. The 3.5 kb transcript is the most predominant and is expressed in all the tissues examined. A cDNA containing a 91 nucleotide insertion at the N‐terminal region of huCDC7 is also detected, suggesting the presence of multiple splicing variants. The huCdc7 protein is expressed at a constant level during the mitotic cell cycle and is localized primarily in nuclei in interphase and distributed diffusibly in cytoplasm in the mitotic phase. The wild‐type huCdc7 protein expressed in COS7 cells phosphorylates MCM2 and MCM3 proteins in vitro, suggesting that huCdc7 may regulate processes of DNA replication by modulating MCM functions.
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酿酒酵母 CDC7 在细胞周期中功能的调节。
影响因子：
3.3
作者：
Yoon,HJ;Loo,S;Campbell,JL
通讯作者：
Campbell,JL
影响因子：
56.9
作者：
MARAHRENS, Y;STILLMAN, B
通讯作者：
STILLMAN, B
影响因子：
56.9
作者：
BELL, SP;KOBAYASHI, R;STILLMAN, B
通讯作者：
STILLMAN, B
影响因子：
10.5
作者：
FOX, CA;LOO, S;RINE, J
通讯作者：
RINE, J
影响因子：
10.5
作者：
Hong Yan;Ankit Margaret Merchant;B. Tye
通讯作者：
Hong Yan;Ankit Margaret Merchant;B. Tye