Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration.
Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration.
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DOI：
10.1371/journal.pone.0169522
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Ohno J
中科院分区：
文献类型：
--
作者：
Sato A;Kajiya H;Mori N;Sato H;Fukushima T;Kido H;Ohno J
骨再生的初始步骤需要成骨细胞迁移到缺损部位。我们以前的研究表明，鲑鱼DNA为基础的支架可以促进大鼠颅骨缺损的骨再生。我们推测鲑鱼DNA可能具有骨诱导特性，包括迁移成骨细胞的归巢。在本研究中，我们研究了鲑鱼DNA对成骨细胞分化和诱导成骨细胞迁移的影响，使用MG 63细胞（人前成骨细胞）在体外。此外，我们分析了一个临界大小的在体大鼠颅骨骨缺损（CSD）模型的骨再生。与单独使用成骨诱导培养基的培养相比，鲑鱼DNA增强了MG 63细胞中成骨相关因子、侏儒相关转录因子2（Runx 2）、碱性磷酸酶和osterix（OSX）的mRNA和蛋白质表达。从组织化学和免疫组织化学分析，使用冷冻切片的骨缺损的动物植入DNA磁盘，许多细胞被发现表达醛脱氢酶1，间充质干细胞的标志物之一。另外，骨缺损的替代结缔组织中观察到OSX。这些发现表明DNA诱导成骨细胞向再生组织的迁移和积累。此外，体外transwell迁移试验表明，与单独的培养基相比，DNA的加入增强了成骨细胞迁移的诱导。植入的DNA磁盘促进大鼠CSD的骨再生，与胶原磁盘相比。这些结果表明，鲑鱼DNA增强成骨细胞分化和诱导迁移，从而促进骨再生。
The initial step of bone regeneration requires the migration of osteogenic cells to defective sites. Our previous studies suggest that a salmon DNA-based scaffold can promote the bone regeneration of calvarial defects in rats. We speculate that the salmon DNA may possess osteoinductive properties, including the homing of migrating osteogenic cells. In the present study, we investigated the influence of the salmon DNA on osteoblastic differentiation and induction of osteoblast migration using MG63 cells (human preosteoblasts) in vitro. Moreover, we analyzed the bone regeneration of a critical-sized in vivo calvarial bone defect (CSD) model in rats. The salmon DNA enhanced both mRNA and protein expression of the osteogenesis-related factors, runt-related transcription factor 2 (Runx2), alkaline phosphatase, and osterix (OSX) in the MG63 cells, compared with the cultivation using osteogenic induction medium alone. From the histochemical and immunohistochemical assays using frozen sections of the bone defects from animals that were implanted with DNA disks, many cells were found to express aldehyde dehydrogenase 1, one of the markers for mesenchymal stem cells. In addition, OSX was observed in the replaced connective tissue of the bone defects. These findings indicate that the DNA induced the migration and accumulation of osteogenic cells to the regenerative tissue. Furthermore, an in vitro transwell migration assay showed that the addition of DNA enhanced an induction of osteoblast migration, compared with the medium alone. The implantation of the DNA disks promoted bone regeneration in the CSD of rats, compared with that of collagen disks. These results indicate that the salmon DNA enhanced osteoblastic differentiation and induction of migration, resulting in the facilitation of bone regeneration.
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影响因子：
3.7
作者：
Rangrez AY;M'Baya-Moutoula E;Metzinger-Le Meuth V;Hénaut L;Djelouat MS;Benchitrit J;Massy ZA;Metzinger L
通讯作者：
Metzinger L
影响因子：
2.5
作者：
Fukushima, Tadao;Ohno, Jun;Mitarai, Makoto
通讯作者：
Mitarai, Makoto
DOI：
10.1002/jbm.b.31451
发表时间：
2009-11-01
影响因子：
3.4
作者：
Fukushima, Tadao;Ohno, Jun;Okahata, Yoshio
通讯作者：
Okahata, Yoshio
DOI：
10.1196/annals.1368.026
发表时间：
2006-01-01
期刊：
CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM IV
影响因子：
--
作者：
Tamkovich, Svetlana N.;Cherepanova, Anna V.;Laktionov, Pavel P.
通讯作者：
Laktionov, Pavel P.
碱性磷酸酶在成骨的作用。
影响因子：
15.3
作者：
SIFFERT, R S
通讯作者：
SIFFERT, R S