Identification of two candidate innate immune genes by transcriptional profiling and RNA interference in mouse mammary gland epithelial cells stimulated with lipopolysaccharide
Identification of two candidate innate immune genes by transcriptional profiling and RNA interference in mouse mammary gland epithelial cells stimulated with lipopolysaccharide
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通过脂多糖刺激的小鼠乳腺上皮细胞中的转录谱和 RNA 干扰鉴定两个候选先天免疫基因
DOI：
10.1080/08923973.2016.1222618
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发表时间：
2016-08
影响因子：
3.3
通讯作者：
Lu Wang
中科院分区：
文献类型：
--
作者：
Yu-kun Wang;Jiang Feng;Yu-hao Wei;Lu Wang
摘要乳腺上皮细胞(MECs)在免疫反应和乳房炎等炎症性疾病中发挥重要作用，其机制主要是Toll样受体的激活和细胞因子的释放。然而，微血管内皮细胞对微生物因子刺激的基因表达和生物学途径的整体变化尚不清楚。在此，我们分析了脂多糖作用24 h的小鼠微血管内皮细胞的基因表达谱，微阵列分析显示约有1548个基因差异表达，这些基因主要涉及346个基因本体论术语和128个分子通路，特别是一些与先天免疫相关的通路。通过分析通路关系网络的数据，我们优先考虑了与内毒素相关的差异表达基因。这些变化的重要性得到验证，表明RNA干扰分别抑制了本分析中发现的两个基因--转化生长因子-β1(转化生长因子-β1)和血小板衍生生长因子B(PDGfb)，减少了白细胞介素6(IL-6)和肿瘤坏死因子α的产生。这些发现描绘了脂多糖诱导的小鼠微血管内皮细胞的基因应答模式，并鉴定了与天然免疫密切相关的转化生长因子-β-1和Pdgfb。
Abstract Mammary epithelial cells (MECs) play an important role in immune responses and inflammatory diseases such as mastitis, which is mainly attributed to the activation of Toll-like receptors and the release of cytokines. However, the overall change of gene expression and biological pathways of MECs to microbial factors stimulation remains unknown. Here, we analyzed the gene expression profile in mouse MECs treated with lipopolysaccharide (LPS) for 24 h. Microarray analysis revealed that about 1548 genes differentially expressed, these genes mainly involved in 346 gene ontology terms and 128 molecular pathways, and particularly, some innate immune-associated pathways were significant. By analyzing data for pathway relation network, we prioritized differentially expressed genes with respect to LPS. The importance of changes, indicating that RNA interference-mediated inhibition of two genes identified in this analysis, transforming growth factor beta 1 (Tgf-β1) and platelet-derived growth factor B (Pdgfb), reduced interleukin (IL)-6 and tumor necrosis factor α production respectively, in gene expression was verified. These findings delineate mouse MECs gene response patterns induced by LPS and identify Tgf-β1 and Pdgfb that have been closely related to innate immunity.
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影响因子：
2.4
作者：
R. Pareek;O. Wellnitz;R. van Dorp;J. Burton;D. Kerr
通讯作者：
R. Pareek;O. Wellnitz;R. van Dorp;J. Burton;D. Kerr
影响因子：
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作者：
Im, Jintaek;Lee, Taeheon;Han, Seung Hyun
通讯作者：
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影响因子：
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作者：
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通讯作者：
Hume, David A.
影响因子：
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作者：
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通讯作者：
Li, Jian
影响因子：
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作者：
C. He;Qiong Yi;Yuanfang Li;Hang Yang;Lu Wang
通讯作者：
C. He;Qiong Yi;Yuanfang Li;Hang Yang;Lu Wang