Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases.
Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases.
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DOI：
10.1039/d1gc04519a
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发表时间：
2022-06-20
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影响因子：
--
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中科院分区：
文献类型：
--
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Wood-feeding termites effectively degrade plant biomass through enzymatic degradation. Despite their high efficiencies, however, individual glycoside hydrolases isolated from termites and their symbionts exhibit anomalously low effectiveness in lignocellulose degradation, suggesting hereto unknown enzymatic activities in their digestome. Herein, we demonstrate that an ancient redox-active enzyme encoded by the lower termite Coptotermes gestroi, a Cu/Zn superoxide dismutase (CgSOD-1), plays a previously unknown role in plant biomass degradation. We show that CgSOD-1 transcripts and peptides are up-regulated in response to an increased level of lignocellulose recalcitrance and that CgSOD-1 localizes in the lumen of the fore- and midguts of C. gestroi together with termite main cellulase, CgEG-1-GH9. CgSOD-1 boosts the saccharification of polysaccharides by CgEG-1-GH9. We show that the boosting effect of CgSOD-1 involves an oxidative mechanism of action in which CgSOD-1 generates reactive oxygen species that subsequently cleave the polysaccharide. SOD-type enzymes constitute a new addition to the growing family of oxidases, ones which are up-regulated when exposed to recalcitrant polysaccharides, and that are used by Nature for biomass degradation. A Cu/Zn superoxide dismutase isolated from termite gut can oxidise glucose polymers, boosting the activity of glycoside hydrolases.
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影响因子：
16.6
作者：
Mallinson SJB;Machovina MM;Silveira RL;Garcia-Borràs M;Gallup N;Johnson CW;Allen MD;Skaf MS;Crowley MF;Neidle EL;Houk KN;Beckham GT;DuBois JL;McGeehan JE
通讯作者：
McGeehan JE
DOI：
10.1073/pnas.1323629111
发表时间：
2014-04-29
影响因子：
11.1
作者：
Agger, Jane W.;Isaksen, Trine;Westereng, Bjorge
通讯作者：
Westereng, Bjorge
影响因子：
6.3
作者：
Filiatrault-Chastel, Camille;Navarro, David;Berrin, Jean-Guy
通讯作者：
Berrin, Jean-Guy
影响因子：
5.9
作者：
Calusinska, Magdalena;Marynowska, Martyna;Delfosse, Philippe
通讯作者：
Delfosse, Philippe
影响因子：
5
作者：
KONINGSBERGER, JC;VANASBECK, BS;MARX, JJM
通讯作者：
MARX, JJM