Screening and Identification of Differential Ovarian Proteins before and after Induced Ovulation via Seminal Plasma in Bactrian Camels.
Screening and Identification of Differential Ovarian Proteins before and after Induced Ovulation via Seminal Plasma in Bactrian Camels.
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双峰驼促排卵前后卵巢差异蛋白的筛选与鉴定
DOI：
10.3390/ani11123512
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发表时间：
2021-12-09
期刊：
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Wang Q;Zhang Q;Li Y;Zhao X;Zhang Y
骆驼是一种诱导排卵动物，其排卵受多种因素的密切调控。了解卵泡发育、激素分泌和排卵的生物学机制需要研究这些机制中涉及的潜在分子途径。然而，对双峰驼中这些分子通路知之甚少。为了筛选和鉴定精浆（SP）诱导卵巢排卵后的候选生物标志物，我们对肌肉注射精浆或磷酸盐缓冲盐水的骆驼卵巢进行了全面的蛋白质组学和分子生物学分析。这些候选生物标志物的鉴定将使人们能够更好地了解双峰驼的繁殖。我们的研究结果为进一步研究诱导排卵的分子机制提供了候选蛋白。骆驼科骆驼属诱导排卵动物，其排卵受多种因素的密切调控。要了解卵泡发育、激素分泌和排卵的生物学机制，需要研究潜在的分子途径。然而，对双峰驼中的这些途径知之甚少。为了筛选和鉴定诱导排卵后的候选生物标志物，本研究对两组骆驼（n = 6）的卵巢进行了全面的蛋白质组学和分子生物学分析。我们鉴定了5075种表达的卵巢蛋白，其中404种在血浆诱导与对照骆驼的样品中差异表达（264种上调，140种下调）（p < 0.05或p < 0.01）。基因本体论注释确定差异表达蛋白（DEP）的潜在功能。这些结果使用蛋白质印迹（p < 0.05）和免疫荧光染色验证了这些蛋白质的子集的差异表达。三种DEP（FST，NR 5A 1和PRL）参与神经化学信号转导，以及内分泌和生殖激素调节过程。《京都基因与基因组百科全书》分析表明，包括钙、cAMP、促性腺激素释放激素、MAPK和神经活性配体-受体信号通路在内的几种通路参与诱导排卵，这表明诱导排卵依赖于下丘脑-垂体-卵巢轴。识别这些候选生物标志物可以更好地了解双峰驼繁殖。卵巢蛋白质组分析和使用更有针对性的方法测量选定的蛋白质是研究诱导排卵机制的一种有前途的方法。
Simple Summary Camelidae are induced ovulators whose ovulation is tightly regulated by multiple factors. Understanding the biological mechanisms underlying follicular development, hormone secretion, and ovulation requires investigating the potential molecular pathways involved in these mechanisms. However, little is known about these molecular pathways in Bactrian camels. To screen and identify candidate biomarkers after seminal plasma (SP)-induced ovulation in the ovaries, we performed comprehensive proteomic and molecular biological analyses of the ovaries from camels that were intramuscularly injected with either seminal plasma or phosphate-buffered saline. Identification of these candidate biomarkers will enable a better understanding of reproduction in Bactrian camels. Our findings suggest candidate proteins for further studies on the molecular mechanisms of induced ovulation. Abstract Camelidae are induced ovulators whose ovulation is tightly regulated by multiple factors. Understanding the biological mechanisms underlying follicular development, hormone secretion, and ovulation requires investigating the potential molecular pathways involved. However, little is known about these pathways in Bactrian camels. To screen and identify candidate biomarkers after inducing ovulation, this study performed comprehensive proteomic and molecular biological analyses of the ovaries from two camel groups (n = 6). We identified 5075 expressed ovarian proteins, of which 404 were differentially expressed (264 upregulated, 140 downregulated) (p < 0.05 or p < 0.01), in samples from plasma-induced versus control camels. Gene ontology annotation identified the potential functions of the differentially expressed proteins (DEPs). These results validated the differential expression for a subset of these proteins using Western blot (p < 0.05) and immunofluorescence staining. Three DEPs (FST, NR5A1, and PRL) were involved in neurochemical signal transduction, as well as endocrine and reproductive hormone regulatory processes. The Kyoto Encyclopedia of Genes and Genomes analysis indicated the involvement of several pathways, including the calcium, cAMP, gonadotropin-releasing hormone, MAPK, and neuroactive ligand–receptor signaling pathways, suggesting that induced ovulation depends on the hypothalamic–pituitary–ovarian axis. Identifying these candidate biomarkers enables a better understanding of Bactrian camel reproduction. Ovarian proteomic profiling and the measurement of selected proteins using more targeted methods is a promising approach for studying induced-ovulation mechanisms.
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DOI：
10.1056/nejmoa0806228
发表时间：
2009-03-19
期刊：
The New England journal of medicine
影响因子：
--
作者：
Lourenço D;Brauner R;Lin L;De Perdigo A;Weryha G;Muresan M;Boudjenah R;Guerra-Junior G;Maciel-Guerra AT;Achermann JC;McElreavey K;Bashamboo A
通讯作者：
Bashamboo A
影响因子：
4.1
作者：
GREENWOOD, FC;HUNTER, WM
通讯作者：
HUNTER, WM
影响因子：
3.6
作者：
Adams, GP;Ratto, MH;Singh, J
通讯作者：
Singh, J
影响因子：
2.7
作者：
Daza, Daniel Ocampo;Larhammar, Dan
通讯作者：
Larhammar, Dan
DOI：
10.1159/000152036
发表时间：
2008
期刊：
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation
影响因子：
--
作者：
Lin L;Achermann JC
通讯作者：
Achermann JC